中华实验和临床感染病杂志（电子版）
中華實驗和臨床感染病雜誌（電子版）
중화실험화림상감염병잡지（전자판）
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL INFECTIOUS DISEASES(ELECTRONIC VERSION)
2013年
6期
821-824
,共4页
邹美银%朱勇根%汪美华%吴月平%俞冲
鄒美銀%硃勇根%汪美華%吳月平%俞遲
추미은%주용근%왕미화%오월평%유충
肝细胞癌%白细胞介素-32,金属蛋白酶-2
肝細胞癌%白細胞介素-32,金屬蛋白酶-2
간세포암%백세포개소-32,금속단백매-2
Primary hepatocellur carcinoma%Interleukin-32%Matrix metalloproteases-2
目的：探讨白细胞介素（IL）-32和基质金属蛋白酶（MMP）-2对原发性肝细胞癌（PHC）的临床诊断价值。方法选择38例PHC及32例肝硬化患者作为研究对象，采用酶联免疫吸附试验检测血清MMP-2和IL-32表达水平，分析其与甲胎蛋白（AFP）的相关性及对PHC的诊断价值。结果 PHC患者血清MMP-2表达水平显著高于肝硬化及健康对照组（t =4.012、3.707，P =0.000、0.001）；PHC患者血清IL-32表达水平高于肝硬化及健康对照组（t =3.265、9.001，P =0.002、0.000），肝硬化患者高于健康对照组（t =6.876，P =0.000）；血清MMP-2、IL-32和AFP预测PHC发生ROC面积为0.827、0.702和0.873；三者均能较好地预测PHC发生（P均＜0.05）；以血清MMP-2、IL-32和AFP为参数，拟合预测PHC发生的Logistic二元回归方程，模型方程拟合优度高（χ2=0.714，P =0.994），准确率达到89.47%（34/38）。结论 IL-32、MMP-2和AFP均能较好地预测PHC的发生，若三者联合作为预测因素，则准确性更高。
目的：探討白細胞介素（IL）-32和基質金屬蛋白酶（MMP）-2對原髮性肝細胞癌（PHC）的臨床診斷價值。方法選擇38例PHC及32例肝硬化患者作為研究對象，採用酶聯免疫吸附試驗檢測血清MMP-2和IL-32錶達水平，分析其與甲胎蛋白（AFP）的相關性及對PHC的診斷價值。結果 PHC患者血清MMP-2錶達水平顯著高于肝硬化及健康對照組（t =4.012、3.707，P =0.000、0.001）；PHC患者血清IL-32錶達水平高于肝硬化及健康對照組（t =3.265、9.001，P =0.002、0.000），肝硬化患者高于健康對照組（t =6.876，P =0.000）；血清MMP-2、IL-32和AFP預測PHC髮生ROC麵積為0.827、0.702和0.873；三者均能較好地預測PHC髮生（P均＜0.05）；以血清MMP-2、IL-32和AFP為參數，擬閤預測PHC髮生的Logistic二元迴歸方程，模型方程擬閤優度高（χ2=0.714，P =0.994），準確率達到89.47%（34/38）。結論 IL-32、MMP-2和AFP均能較好地預測PHC的髮生，若三者聯閤作為預測因素，則準確性更高。
목적：탐토백세포개소（IL）-32화기질금속단백매（MMP）-2대원발성간세포암（PHC）적림상진단개치。방법선택38례PHC급32례간경화환자작위연구대상，채용매련면역흡부시험검측혈청MMP-2화IL-32표체수평，분석기여갑태단백（AFP）적상관성급대PHC적진단개치。결과 PHC환자혈청MMP-2표체수평현저고우간경화급건강대조조（t =4.012、3.707，P =0.000、0.001）；PHC환자혈청IL-32표체수평고우간경화급건강대조조（t =3.265、9.001，P =0.002、0.000），간경화환자고우건강대조조（t =6.876，P =0.000）；혈청MMP-2、IL-32화AFP예측PHC발생ROC면적위0.827、0.702화0.873；삼자균능교호지예측PHC발생（P균＜0.05）；이혈청MMP-2、IL-32화AFP위삼수，의합예측PHC발생적Logistic이원회귀방정，모형방정의합우도고（χ2=0.714，P =0.994），준학솔체도89.47%（34/38）。결론 IL-32、MMP-2화AFP균능교호지예측PHC적발생，약삼자연합작위예측인소，칙준학성경고。
Objective To explore the clinical value of interleukin (IL)-32, matrix metalloproteases (MMP) -2 for the diagnosis of primary hepatocellular carcinoma (PHC). Methods Total of 38 patients with PHC, 32 patients with liver cirrhosis and 20 healthy controls were enrolled in the study. IL-32, MMP-2 were detected by ELISA. The relationship between IL-32, MMP-2 and AFP and their predicting value for PHC were analyzed, respectively. Results The concentration of MMP-2 in the serum of patients with PHC was higher than that in patients with liver cirrhosis and healthy controls (t = 4.012, 3.707; P = 0.000, 0.001). The concentration of IL-32 in the serum of patients with PHC was higher than that in patients with liver cirrhosis and healthy controls (t = 3.265, 9.001; P = 0.002, 0.000), and that in patients with liver cirrhosis was higher than in healthy controls (t = 6.876, P = 0.000). The area under the ROC cures of MMP-2, IL-32, AFP in predicting PHC were 0.827, 0.702 and 0.873, respectively (P < 0.05). If taking MMP-2, IL-32 and AFP as parameter to predict occurrence of PHC, the logistic binary regression equation had high goodness-of-fit (χ2= 0.714, P = 0.909), the accuracy was 89.47% (34/38). Conclusions MMP-2, IL-32 and AFP could predict occurrence of PHC, if taken as predictors together, would get very high accuracy.
