CAJ | 학술논문

目的：探讨黄芩对大鼠肝硬化内毒素血症肠黏膜损伤的保护性机制。方法复合因素造模法建立肝硬化内毒素血症大鼠模型。模型确立后将大鼠随机分为3个实验组，即模型组、黄芩组治疗组和谷氨酰胺治疗组，每组各20只；另设正常对照组大鼠10只。各组大鼠给予灌胃治疗，共2周。实验结束后，采用ELISA法检测大鼠血清内毒素水平，TUNEL法检测各组大鼠肠黏膜细胞凋亡并计算凋亡率，采用RT-PCR检测肠黏膜细胞凋亡相关基因Bcl-2 RNA和Bax RNA的表达水平。结果3个实验组较正常对照组大鼠内毒素组水平均显著升高（F =3.31，P ＜0.05）；其中模型组显著高于黄芩治疗组（q =5.12，P =0.0000）；黄芩治疗组显著低于谷氨酰胺组（q =3.74，P =0.0123）。3个实验组与正常对照组比较，肠黏膜细胞的凋亡率均显著升高（F =4.77，P ＜0.01）；其中模型组显著高于黄芩治疗组和谷氨酰胺组（q =4.56、4.35，P均＜0.01）；黄芩治疗组显著低于谷氨酰胺组（q =3.78，P =0.012）。3个实验组与正常对照组比较，肠黏膜组织Bcl-2 mRNA水平显著下降（F =3.55， P ＜0.05）；其中模型组显著低于黄芩治疗组和谷氨酰胺治疗组（q =3.89、3.40，P ＜0.05）；黄芩治疗组显著高于谷氨酰胺组（q =2.77，P ＜0.05）。3个实验组大鼠肠黏膜Bax mRNA水平则显著高于正常对照组（F =3.67，P ＜0.05）；模型组显著高于黄芩治疗组和谷氨酰胺治疗组（q =3.62、2.91，P ＜0.05）；黄芩治疗组显著低于谷氨酰胺组（q =2.85，P ＜0.05）。结论黄芩能够通过降低肠黏膜细胞的凋亡而减少肝硬化内毒素血症的发生。
목적：탐토황금대대서간경화내독소혈증장점막손상적보호성궤제。방법복합인소조모법건립간경화내독소혈증대서모형。모형학립후장대서수궤분위3개실험조，즉모형조、황금조치료조화곡안선알치료조，매조각20지；령설정상대조조대서10지。각조대서급여관위치료，공2주。실험결속후，채용ELISA법검측대서혈청내독소수평，TUNEL법검측각조대서장점막세포조망병계산조망솔，채용RT-PCR검측장점막세포조망상관기인Bcl-2 RNA화Bax RNA적표체수평。결과3개실험조교정상대조조대서내독소조수평균현저승고（F =3.31，P ＜0.05）；기중모형조현저고우황금치료조（q =5.12，P =0.0000）；황금치료조현저저우곡안선알조（q =3.74，P =0.0123）。3개실험조여정상대조조비교，장점막세포적조망솔균현저승고（F =4.77，P ＜0.01）；기중모형조현저고우황금치료조화곡안선알조（q =4.56、4.35，P균＜0.01）；황금치료조현저저우곡안선알조（q =3.78，P =0.012）。3개실험조여정상대조조비교，장점막조직Bcl-2 mRNA수평현저하강（F =3.55， P ＜0.05）；기중모형조현저저우황금치료조화곡안선알치료조（q =3.89、3.40，P ＜0.05）；황금치료조현저고우곡안선알조（q =2.77，P ＜0.05）。3개실험조대서장점막Bax mRNA수평칙현저고우정상대조조（F =3.67，P ＜0.05）；모형조현저고우황금치료조화곡안선알치료조（q =3.62、2.91，P ＜0.05）；황금치료조현저저우곡안선알조（q =2.85，P ＜0.05）。결론황금능구통과강저장점막세포적조망이감소간경화내독소혈증적발생。
Objective To explore the protective mechanism of Scutellaria baicalensis georgi on the intestinal mucosal injury of endotoxemia in rats with liver cirrhosis. Methods Compound factors modeling was used to establish endotoxemia rat model with liver cirrhosis. The rats were randomly divided into 3 experimental groups: model group, the scutellaria treatment group and glutamine treatment group with 20 in each group. There were 10 rats in normal control group. Gavage was conducted to each group for 2 weeks. At the end of the experiment, serum endotoxin levels of rats and intestinal mucosa apoptosis of rats in each group were detected by ELISA and TUNEL, respectively. And the expression levels of gene Bcl-2 RNA and Bax RNA related to intestinal mucosa apoptosis were detected by RT-PCR. Results The endotoxin level of the three experimental groups was significantly elevated compared with that of the normal control group (F = 3.31, P < 0.05). The endotoxin level of the model group was significantly higher than that of the scutellaria treatment group (q = 5.12, P = 0.0000 ). The endotoxin level of the scutellaria treatment group was significantly lower than that of the glutamine group (q = 3.74, P = 0.0123). The increase of intestinal mucosal apoptosis rate in the three experimental groups was significantly higher than that of the normal control group (F = 4.77, P < 0.01). The intestinal mucosal apoptosis rate of the model group was significantly higher than that of the scutellaria treatment group and the glutamine group (q = 4.56, 4.35; P = 0.0000). The rate of intestinal mucosal apoptosis in the scutellaria treatment group was significantly lower than that of the glutamine group (q = 3.78, P = 0.012). The intestinal mucosal Bcl-2 mRNA level of the three experimental groups significantly decreased than that of the normal control group (F = 3.55, P < 0.05). The intestinal mucosal Bcl-2 mRNA levels of the model group were significantly lower than that of the scutellaria treatment group and the glutamine treatment group (q = 3.89, 3.40; P < 0.05). The intestinal mucosal Bcl-2 mRNA level of the scutellaria treatment group was significantly higher than that of the glutamine group (q = 2.77, P <0.05). The intestinal mucosal Bax mRNA level of the three experimental groups was significantly higher than that of the normal control group (F = 3.67, P < 0.05). The intestinal mucosal Bax mRNA level of the model group was significantly higher than that of the scutellaria treatment group and the glutamine treatment group (q = 3.62, 2.91; P < 0.05 ). The intestinal mucosal Bax mRNA level of the scutellaria treatment group was significantly lower than that of the glutamine group (q = 2.85,P < 0.05). Conclusions Scutellaria baicalensis georgi could reduce the occurrence of liver cirrhosis endotoxemia by reducing intestinal mucosal apoptosis.