医学检验与临床
醫學檢驗與臨床
의학검험여림상
MEDICAL LATORATORY SCIENCE AND CLINICES
2013年
6期
4-5,3
,共3页
刘宏峰%张彩虹%朱斌
劉宏峰%張綵虹%硃斌
류굉봉%장채홍%주빈
肿瘤相关巨噬细胞%肝癌细胞系%转移
腫瘤相關巨噬細胞%肝癌細胞繫%轉移
종류상관거서세포%간암세포계%전이
Tumor-associated macrophage%Hepatocellular carcinoma cell lines%Metastasis
目的:探讨肿瘤相关巨噬细胞对肝癌细胞系细胞转移与侵袭力的影响。方法:巨噬细胞加入佛波酯进行刺激培养,体外模拟肿瘤相关巨噬细胞的体内环境,然后把其上清加入肝癌细胞系的transwell小室中去,观察其对肝癌细胞迁移的影响。结果:活化的巨噬细胞上清可对肝癌细胞系的迁移产生明显影响。结论:肿瘤相关巨噬细胞可能参与了肝癌细胞的体内转移过程。
目的:探討腫瘤相關巨噬細胞對肝癌細胞繫細胞轉移與侵襲力的影響。方法:巨噬細胞加入彿波酯進行刺激培養,體外模擬腫瘤相關巨噬細胞的體內環境,然後把其上清加入肝癌細胞繫的transwell小室中去,觀察其對肝癌細胞遷移的影響。結果:活化的巨噬細胞上清可對肝癌細胞繫的遷移產生明顯影響。結論:腫瘤相關巨噬細胞可能參與瞭肝癌細胞的體內轉移過程。
목적:탐토종류상관거서세포대간암세포계세포전이여침습력적영향。방법:거서세포가입불파지진행자격배양,체외모의종류상관거서세포적체내배경,연후파기상청가입간암세포계적transwell소실중거,관찰기대간암세포천이적영향。결과:활화적거서세포상청가대간암세포계적천이산생명현영향。결론:종류상관거서세포가능삼여료간암세포적체내전이과정。
Objective:To investigate the effects of tumor-associated macrophage on metastasis and invasive ability of hepatocellular carcinoma cell lines . Methods:Macrophages were treated with PMA to mimic the tumor-associated macrophages environment in vitro , and then adding the supernatant to hepatoma cell lines in the transwell chamber, to observe its effect on migration of hepatocellular carcinoma cell line.Results:Supernatant of activated macrophages has a significant impact on the migration of hepatocellular carcinoma cell line. Conclusions:Tumor-associated macrophage may be involved in the process of metastasis and invasive ability of hepatoma cells in vivo.