中国医药
中國醫藥
중국의약
CHINA MEDICINE
2014年
5期
722-725
,共4页
王治洁%应小燕%贾素娟%高菲菲
王治潔%應小燕%賈素娟%高菲菲
왕치길%응소연%가소연%고비비
胎膜早破%基质金属蛋白酶9%金属蛋白酶组织抑制剂2%核因子-κB
胎膜早破%基質金屬蛋白酶9%金屬蛋白酶組織抑製劑2%覈因子-κB
태막조파%기질금속단백매9%금속단백매조직억제제2%핵인자-κB
Premature rupture of membrane%Matrix metallo proteinases 9%Tissue inhibitor of matrix metallo proteinase 2%Nuclear factor Kappa B
目的 探讨胎膜早破患者胎盘胎膜组织中基质金属蛋白酶9(MMP-9)、金属蛋白酶组织抑制剂2(TIMP-2)和核因子κB(NF-κB)表达变化及意义.方法 采用免疫组织化学法,实时定量聚合酶链反应法和免疫印迹法分别检测胎膜早破剖宫产病例45例(胎膜早破组)、正常妊娠择期剖宫产52例(未临产组)、足月自然临产组56例(临产组)孕妇分娩后胎膜组织中MMP-9、TIMP-2和NF-κB的表达水平.结果 实时定量PCR在未临产组,临产组和胎膜早破组中,MMP-9的相对表达量分别是(3.0±1.2)、(10.4±3.1)、(22.1±3.5),TIMP-2的相对表达量分别是(7.1±2.7)、(4.6±2.0)、(3.6±1.8),NF-κBP65在3组的相对表达量分别是(1.1±0.4)、(2.5±0.4)、(3.3±0.4),3组数据比较差异均有统计学意义(P＜0.05);免疫印迹显示,未临产、临产和胎膜早破组的MMP-9在胎膜的IOD值分别为(29.5±1.2)、(68.7±3.1)、(167.2±4.8),TIMP-1的IOD值分别是(61.1±4.1),(32.2±2.7),(7.3±3.0),NF-κB P65的IOD值分别是(22.3±3.2)、(52.5±3.6)、(182.7±5.0),3组数据比较差异均有统计学意义(P＜0.05).结论 MMP-9在胎膜早破中起到关键性作用,MMP-9/TIMP-2的平衡失调可能间接导致胎膜早破,可能通过NF-κB信号通路发挥调控作用.
目的 探討胎膜早破患者胎盤胎膜組織中基質金屬蛋白酶9(MMP-9)、金屬蛋白酶組織抑製劑2(TIMP-2)和覈因子κB(NF-κB)錶達變化及意義.方法 採用免疫組織化學法,實時定量聚閤酶鏈反應法和免疫印跡法分彆檢測胎膜早破剖宮產病例45例(胎膜早破組)、正常妊娠擇期剖宮產52例(未臨產組)、足月自然臨產組56例(臨產組)孕婦分娩後胎膜組織中MMP-9、TIMP-2和NF-κB的錶達水平.結果 實時定量PCR在未臨產組,臨產組和胎膜早破組中,MMP-9的相對錶達量分彆是(3.0±1.2)、(10.4±3.1)、(22.1±3.5),TIMP-2的相對錶達量分彆是(7.1±2.7)、(4.6±2.0)、(3.6±1.8),NF-κBP65在3組的相對錶達量分彆是(1.1±0.4)、(2.5±0.4)、(3.3±0.4),3組數據比較差異均有統計學意義(P＜0.05);免疫印跡顯示,未臨產、臨產和胎膜早破組的MMP-9在胎膜的IOD值分彆為(29.5±1.2)、(68.7±3.1)、(167.2±4.8),TIMP-1的IOD值分彆是(61.1±4.1),(32.2±2.7),(7.3±3.0),NF-κB P65的IOD值分彆是(22.3±3.2)、(52.5±3.6)、(182.7±5.0),3組數據比較差異均有統計學意義(P＜0.05).結論 MMP-9在胎膜早破中起到關鍵性作用,MMP-9/TIMP-2的平衡失調可能間接導緻胎膜早破,可能通過NF-κB信號通路髮揮調控作用.
목적 탐토태막조파환자태반태막조직중기질금속단백매9(MMP-9)、금속단백매조직억제제2(TIMP-2)화핵인자κB(NF-κB)표체변화급의의.방법 채용면역조직화학법,실시정량취합매련반응법화면역인적법분별검측태막조파부궁산병례45례(태막조파조)、정상임신택기부궁산52례(미임산조)、족월자연임산조56례(임산조)잉부분면후태막조직중MMP-9、TIMP-2화NF-κB적표체수평.결과 실시정량PCR재미임산조,임산조화태막조파조중,MMP-9적상대표체량분별시(3.0±1.2)、(10.4±3.1)、(22.1±3.5),TIMP-2적상대표체량분별시(7.1±2.7)、(4.6±2.0)、(3.6±1.8),NF-κBP65재3조적상대표체량분별시(1.1±0.4)、(2.5±0.4)、(3.3±0.4),3조수거비교차이균유통계학의의(P＜0.05);면역인적현시,미임산、임산화태막조파조적MMP-9재태막적IOD치분별위(29.5±1.2)、(68.7±3.1)、(167.2±4.8),TIMP-1적IOD치분별시(61.1±4.1),(32.2±2.7),(7.3±3.0),NF-κB P65적IOD치분별시(22.3±3.2)、(52.5±3.6)、(182.7±5.0),3조수거비교차이균유통계학의의(P＜0.05).결론 MMP-9재태막조파중기도관건성작용,MMP-9/TIMP-2적평형실조가능간접도치태막조파,가능통과NF-κB신호통로발휘조공작용.
Objective To discuss the expression and significance of matrix metallo proteinases 9 (MMP-9),tissue inhibitor of matrix metallo proteinase 2 (TIMP-2) and nuclear factor Kappa B (NF-κB) in the fetal membrane of pregnant women with PROM.Methods The expression of MMP-9,TIMP-2 and NF-κB was measured by immunohistochemical method (IHC),realtime polymerase chain reaction(PCR) and Western Blot technique in 45 PROM cases with cesarean section (PROM group),52 normal pregnancy cases with elective cesarean section (not labor onset group) and 56 term labor cases with spontaneous parturient (labor onset group).Results Quantitative PCR showed MMP-9 gene expression in not labor onset group,labor onset group and PROM group was(3.0 ± 1.2),(10.4 ± 3.1),(22.1 ± 3.5) separately.TIMP-2 gene expression in three groups was (7.1 ± 2.7),(4.6 ± 2.0),(3.6 ± 1.8),NF-κB P65 gene expression in the groups was (1.1 ± 0.4),(2.5 ± 0.4),(3.3 ± 0.4),There was statistics difference in groups (P ＜ 0.05) about three gene expression.The IOD value of MMP-9 by Western Blot in three groups was (29.5 ± 1.2),(68.7 ± 3.1),(167.2 ± 4.8).The IOD value of TIMP-2 was (61.1 ± 4.1),(32.2 ± 2.7),(7.3 ± 3.0).The IOD value of NF-κB P65 was (22.3 ± 3.2),(52.5 ± 3.6),(182.7 ± 5.0) in not labor onset group,labor onset group and PROM group separately.There was statistics difference in groups(P ＜ 0.05) about three protein expression.Conclusions The expression of MMP-9 and TIMP-2 is of negative correlation.The proportion between MMP-9 and TIMP-2 is crucial for PROM and it can take effect through NF-κB signaling.
