仲恺农业工程学院学报
仲愷農業工程學院學報
중개농업공정학원학보
JOURNAL OF ZHONGKAI UNIVERSITY OF AGRICULTURE AND TECHNOLOGY
2014年
3期
5-8,13
,共5页
梁丽凤%林晓林%伍宇雁%莫艳坤%周玲艳
樑麗鳳%林曉林%伍宇雁%莫豔坤%週玲豔
량려봉%림효림%오우안%막염곤%주령염
金线莲(Anoectochilus formosanus Hayata)%增殖%生根
金線蓮(Anoectochilus formosanus Hayata)%增殖%生根
금선련(Anoectochilus formosanus Hayata)%증식%생근
Anoectochilus formosanus%proliferation%rooting
为了优化金线莲（ Anoectochilus formosanus Hayata ）增殖和生根培养条件，将金线莲无菌苗的顶芽和带节茎段接种到不同种类和浓度的激素以及添加物组合的培养基中进行增殖和生根培养，以筛选出最适合金线莲增殖和生根的培养基．结果表明，金线莲最佳增殖培养基为MS＋6-BA 3.0 mg／L＋NAA 0.6 mg／L＋玉米素（ Zeat-in， ZT）0.8 mg／L＋香蕉泥100 g／L，顶芽和腋芽的增值倍数分别为3.82和3.13；生根培养基以 MS＋NAA 0.5 mg／L＋IBA 0.5 mg／L最好，生根率达80％．
為瞭優化金線蓮（ Anoectochilus formosanus Hayata ）增殖和生根培養條件，將金線蓮無菌苗的頂芽和帶節莖段接種到不同種類和濃度的激素以及添加物組閤的培養基中進行增殖和生根培養，以篩選齣最適閤金線蓮增殖和生根的培養基．結果錶明，金線蓮最佳增殖培養基為MS＋6-BA 3.0 mg／L＋NAA 0.6 mg／L＋玉米素（ Zeat-in， ZT）0.8 mg／L＋香蕉泥100 g／L，頂芽和腋芽的增值倍數分彆為3.82和3.13；生根培養基以 MS＋NAA 0.5 mg／L＋IBA 0.5 mg／L最好，生根率達80％．
위료우화금선련（ Anoectochilus formosanus Hayata ）증식화생근배양조건，장금선련무균묘적정아화대절경단접충도불동충류화농도적격소이급첨가물조합적배양기중진행증식화생근배양，이사선출최괄합금선련증식화생근적배양기．결과표명，금선련최가증식배양기위MS＋6-BA 3.0 mg／L＋NAA 0.6 mg／L＋옥미소（ Zeat-in， ZT）0.8 mg／L＋향초니100 g／L，정아화액아적증치배수분별위3.82화3.13；생근배양기이 MS＋NAA 0.5 mg／L＋IBA 0.5 mg／L최호，생근솔체80％．
The aseptic plantlets of Anoectochilus formosanus were used as material in this paper .The opti-mum media for proliferation and rooting were screened while the terminal buds and stem with axillary of A.formosanus were inoculated onto medium with different kind and concentration of hormones and addi -tives.The results showed that the medium of MS +6-BA 3 mg/L +NAA 0.6 mg/L +Zeatin ( ZT) 0.8 mg/L+banana 100 g/L was most suitable for proliferation of A.formosanus, and that the rate of proliferation for terminal buds and axillary buds was 3.85 and 3.07 respectively .The medium of MS+NAA 0.5 mg/L+IBA 0.5 mg/L was most suitable for rooting of A.formosanus, and the rate of rooting reached 80%.
