医药前沿
醫藥前沿
의약전연
YIAYAO QIANYAN
2013年
34期
76-77,78
,共3页
胰岛素%细胞活力%视网膜色素上皮细胞
胰島素%細胞活力%視網膜色素上皮細胞
이도소%세포활력%시망막색소상피세포
目的:研究不同浓度胰岛素对高糖培养下视网膜色素上皮细胞增殖活力的影响。方法:体外培养人RPE(ARPE-19),在高糖(30 mmol/L)浓度下给予0U/L,100U/L,101U/L,102U/L,103U/L,104U/L胰岛素作用12h、24h、48h后用MTT法检测各组视网膜色素上皮细胞的增生活力。结果:各浓度胰岛素组在不同观察时间(12小时、24小时、48小时),其吸光度值(OD570)与空白对照组相比,差异无统计学意义(P>0.05),各组吸光度值(OD570)随作用时间的延长而有增加的趋势,差异具有统计学意义(P<0.05)。结论:胰岛素对高糖培养下视网膜色素上皮细胞增殖活力无明显影响。
目的:研究不同濃度胰島素對高糖培養下視網膜色素上皮細胞增殖活力的影響。方法:體外培養人RPE(ARPE-19),在高糖(30 mmol/L)濃度下給予0U/L,100U/L,101U/L,102U/L,103U/L,104U/L胰島素作用12h、24h、48h後用MTT法檢測各組視網膜色素上皮細胞的增生活力。結果:各濃度胰島素組在不同觀察時間(12小時、24小時、48小時),其吸光度值(OD570)與空白對照組相比,差異無統計學意義(P>0.05),各組吸光度值(OD570)隨作用時間的延長而有增加的趨勢,差異具有統計學意義(P<0.05)。結論:胰島素對高糖培養下視網膜色素上皮細胞增殖活力無明顯影響。
목적:연구불동농도이도소대고당배양하시망막색소상피세포증식활력적영향。방법:체외배양인RPE(ARPE-19),재고당(30 mmol/L)농도하급여0U/L,100U/L,101U/L,102U/L,103U/L,104U/L이도소작용12h、24h、48h후용MTT법검측각조시망막색소상피세포적증생활력。결과:각농도이도소조재불동관찰시간(12소시、24소시、48소시),기흡광도치(OD570)여공백대조조상비,차이무통계학의의(P>0.05),각조흡광도치(OD570)수작용시간적연장이유증가적추세,차이구유통계학의의(P<0.05)。결론:이도소대고당배양하시망막색소상피세포증식활력무명현영향。
Objective:To investigate the effect of different concentrations of insulin on the proliferation of high glucose cultured human retinal pigment epithelial cells-19 in vitro. Methods: Human retinal pigment epithelial cells (ARPE-19) were cultured in vitro, exposed to high glucose group, treated with different concentrations of insulin (0U/L、100U/L、101U/L、102U/L、103U/L、104U/L) respectively. cellproliferation was detected by MTT assay after 12, 24 and 48 hours. Results: There was no statistical significant difference among different concentrations of insulin intervention conditions in groups of high glucose compared to controls (P> 0.05).There was a statistical y significant difference with the extension of time (P<0.05). Conclusion: Insulin had no effect on the proliferation of ARPE-19 cells in the high glucose condition.