CAJ | 학술논문

目的：观察局部移植异体诱导多能干细胞（ induced pluripotent stem cell，IPSC）治疗糖尿病足溃疡 SD大鼠的效果及其对外周血血管内皮生长因子（ vascular endot growth factor，VEGF）的影响。方法取 SD 雄性大鼠40只，随机分为 IPSC治疗组和对照组，分别对两组造糖尿病足溃疡模型。造模成功后 IPSC治疗组在溃疡创面创缘肉膜层多点注射 IPSC悬浊液，对照组在溃疡创面创缘肉膜层多点注射等体积 Knockout DMEM 培养液。观察比较两组治疗第1、5、10天溃疡面积及外周血 VEGF水平。结果40只 SD 雄性大鼠均成功造模。两组溃疡面积比较，治疗第1天差异无统计学意义（P﹥0.05）；治疗第5、10天 IPSC治疗组溃疡面积均小于对照组，差异有统计学意义（P ﹤0.05）。两组外周血 VEGF水平比较，治疗第1天差异无统计学意义（ P ﹥0.05）；治疗第5、10天 IPSC 治疗组外周血 VEGF 水平均高于对照组，差异有统计学意义（P﹤0.05）。结论异体移植 IPSC 可促进糖尿病足溃疡 SD 大鼠溃疡愈合及局部新生血管形成。
목적：관찰국부이식이체유도다능간세포（ induced pluripotent stem cell，IPSC）치료당뇨병족궤양 SD대서적효과급기대외주혈혈관내피생장인자（ vascular endot growth factor，VEGF）적영향。방법취 SD 웅성대서40지，수궤분위 IPSC치료조화대조조，분별대량조조당뇨병족궤양모형。조모성공후 IPSC치료조재궤양창면창연육막층다점주사 IPSC현탁액，대조조재궤양창면창연육막층다점주사등체적 Knockout DMEM 배양액。관찰비교량조치료제1、5、10천궤양면적급외주혈 VEGF수평。결과40지 SD 웅성대서균성공조모。량조궤양면적비교，치료제1천차이무통계학의의（P﹥0.05）；치료제5、10천 IPSC치료조궤양면적균소우대조조，차이유통계학의의（P ﹤0.05）。량조외주혈 VEGF수평비교，치료제1천차이무통계학의의（ P ﹥0.05）；치료제5、10천 IPSC 치료조외주혈 VEGF 수평균고우대조조，차이유통계학의의（P﹤0.05）。결론이체이식 IPSC 가촉진당뇨병족궤양 SD 대서궤양유합급국부신생혈관형성。
Objective To observe the curative effect of allogeneic induced pluripotent stem cells( IPSC)in treatment of diabetic foot ulcers in SD rats by local transplantation,and the effect on vascular endothelial growth factor( VEGF). Meth-ods A total of 40 male SD rats were randomly divided into IPSC treatment group(n=20)and control group(n=20). Rat models of diabetic foot ulcers were established in the two groups. In the IPSC treatment group,ulcer wounds were multipointly injected with IPSC suspensions into tunica dartos retia,while the control group was injected with the same volume of Knockout DMEM culture solution. The ulcer areas on the 1st d,5th d and 10th d of treatment and VEGF levels in peripheral blood of the two groups were observed and comparedResults The 40 SD rat models were successfully established. There was no significant difference in ulcer area on the 1st d of treatment in the two groups(P﹥0. 05);ulcer areas in IPSC treatment group on the 5th d and 10th d of treatment were significantly smaller compared with those in control group(P﹤0. 05). There was no significant difference in VEGF level of peripheral blood on the 1st d of treatment in the two groups(P﹥0. 05);levels of peripheral blood in IPSC treatment group on the 5th d and 10th d of treatment were significantly higher than those in control group(P﹤ 0. 05). Conclusion IPSC allotransplantation may promote the healing of diabetic foot ulcers and local neovascularization in SD rats.