CAJ | 학술논문

目的：探讨雌激素对子宫内膜异位症在位内膜间质细胞巨噬细胞移动抑制因子（ MIF）表达的调控作用及其意义。方法：用免疫组化鉴定子宫内膜间质细胞，RT-PCR及蛋白免疫印迹法测定MIF mRNA及蛋白的表达。结果：子宫内膜间质细胞经雌激素作用后分泌MIF mRNA及蛋白水平明显增高。子宫内膜异位症组MIF上调水平明显高于正常组。结论：子宫内膜异位症内膜间质细胞中MIF的表达对雌激素的刺激呈现超敏状态，子宫内膜间质细胞内MIF表达升高，可能促进子宫内膜异位症的发生发展。
목적：탐토자격소대자궁내막이위증재위내막간질세포거서세포이동억제인자（ MIF）표체적조공작용급기의의。방법：용면역조화감정자궁내막간질세포，RT-PCR급단백면역인적법측정MIF mRNA급단백적표체。결과：자궁내막간질세포경자격소작용후분비MIF mRNA급단백수평명현증고。자궁내막이위증조MIF상조수평명현고우정상조。결론：자궁내막이위증내막간질세포중MIF적표체대자격소적자격정현초민상태，자궁내막간질세포내MIF표체승고，가능촉진자궁내막이위증적발생발전。
AIM:To investigate the effects of 17β-estradiol on the expression of macrophage migration inhibi-tory factor ( MIF) in cultured endometrial stromal cells from endometriosis .METHODS:Immunohistochemistry was used to identify the endometrial stromal cells .The expression of MIF at mRNA and protein levels was assessed by RT-PCR and Western blotting .RESULTS:Elevated expression of MIF at mRNA and protein levels was observed in the cultured endom-etrial stromal cells treated with 17β-estradiol.In endometrial stromal cells from the women with endometriosis , the level of MIF up-regulation by 17β-estradiol was significantly higher than that in the cells from the women without endometriosis . CONCLUSION:Endometrial stromal cells from endometriosis are more sensitive to 17β-estradiol, which up-regulates the expression of MIF and contribute to the pathogenesis and progression of endometriosis .