中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2014年
4期
625-628
,共4页
胃肿瘤%葡萄糖调节蛋白78%细胞周期蛋白D1
胃腫瘤%葡萄糖調節蛋白78%細胞週期蛋白D1
위종류%포도당조절단백78%세포주기단백D1
Stomach neoplasms%Glucose-regulated protein 78%Cyclin D1
目的:探讨内质网分子伴侣葡萄糖调节蛋白78( GRP78)参与胃癌细胞生长的作用。方法:回顾性分析34例胃癌及34例癌旁组织,利用组织微阵列技术,构建组织阵列,采用免疫组织化学技术SP法检测该阵列中GRP78蛋白在胃癌及其癌旁组织中表达情况。利用Western blotting 检测人体胃癌组织、癌旁组织(肿瘤旁1 cm处)和正常组织(远离肿瘤≥10 cm 处)中GRP78蛋白的表达情况。利用Western blotting 检测人胃癌细胞SGC7901和过表达GRP78的SGC7901-H78细胞(稳定转染GRP78)中GRP78和生长相关蛋白cyclin D1的表达情况。结果:(1)免疫组织化学检测发现:人体胃癌组织中GRP78表达水平明显高于癌旁组织和正常组织,且与性别、分化程度相关(P<0.05);(2) Western blotting检测发现:胃癌组织中GRP78蛋白的表达明显高于癌旁组织和正常组织;(3) Western blotting检测发现:相对于SGC7901细胞,SGC7901-H78细胞中GRP78蛋白的表达明显升高,同时检测生长相关蛋白cyclin D1的表达发现,随着GRP78表达的上调,cyclin D1蛋白表达增加。结论:GRP78蛋白的高表达可能参与了胃癌细胞的生长。
目的:探討內質網分子伴侶葡萄糖調節蛋白78( GRP78)參與胃癌細胞生長的作用。方法:迴顧性分析34例胃癌及34例癌徬組織,利用組織微陣列技術,構建組織陣列,採用免疫組織化學技術SP法檢測該陣列中GRP78蛋白在胃癌及其癌徬組織中錶達情況。利用Western blotting 檢測人體胃癌組織、癌徬組織(腫瘤徬1 cm處)和正常組織(遠離腫瘤≥10 cm 處)中GRP78蛋白的錶達情況。利用Western blotting 檢測人胃癌細胞SGC7901和過錶達GRP78的SGC7901-H78細胞(穩定轉染GRP78)中GRP78和生長相關蛋白cyclin D1的錶達情況。結果:(1)免疫組織化學檢測髮現:人體胃癌組織中GRP78錶達水平明顯高于癌徬組織和正常組織,且與性彆、分化程度相關(P<0.05);(2) Western blotting檢測髮現:胃癌組織中GRP78蛋白的錶達明顯高于癌徬組織和正常組織;(3) Western blotting檢測髮現:相對于SGC7901細胞,SGC7901-H78細胞中GRP78蛋白的錶達明顯升高,同時檢測生長相關蛋白cyclin D1的錶達髮現,隨著GRP78錶達的上調,cyclin D1蛋白錶達增加。結論:GRP78蛋白的高錶達可能參與瞭胃癌細胞的生長。
목적:탐토내질망분자반려포도당조절단백78( GRP78)삼여위암세포생장적작용。방법:회고성분석34례위암급34례암방조직,이용조직미진렬기술,구건조직진렬,채용면역조직화학기술SP법검측해진렬중GRP78단백재위암급기암방조직중표체정황。이용Western blotting 검측인체위암조직、암방조직(종류방1 cm처)화정상조직(원리종류≥10 cm 처)중GRP78단백적표체정황。이용Western blotting 검측인위암세포SGC7901화과표체GRP78적SGC7901-H78세포(은정전염GRP78)중GRP78화생장상관단백cyclin D1적표체정황。결과:(1)면역조직화학검측발현:인체위암조직중GRP78표체수평명현고우암방조직화정상조직,차여성별、분화정도상관(P<0.05);(2) Western blotting검측발현:위암조직중GRP78단백적표체명현고우암방조직화정상조직;(3) Western blotting검측발현:상대우SGC7901세포,SGC7901-H78세포중GRP78단백적표체명현승고,동시검측생장상관단백cyclin D1적표체발현,수착GRP78표체적상조,cyclin D1단백표체증가。결론:GRP78단백적고표체가능삼여료위암세포적생장。
AIM:To explore the effect of glucose-regulated protein 78 (GRP78) on the gastric carcinogenesis. METHODS:GPR78 expression patterns were examined in 34 specimens from gastric carcinoma patients using the immu-nohistochemistry (IHC) assay, and in 10 specimens using Western blotting analysis .In addition, the expression of GPR78 and cyclin D1 was detected in human gastric cancer cell lines SGC 7901 and SGC7901-H78 (overexpressing GRP78) by Western blotting.RESULTS:By IHC assay, GRP78 was found to be highly expressed in the cytoplasm of gastric carcino-mas as compared with the adjacent non-malignant tissues and corresponding normal tissues .GRP78 expression was positive-ly correlated with gender and histological differentiation (P<0.05), but not with age, tumor stage and lymph node metas-tasis (P>0.05).Furthermore, we found that with the increased expression of GRP 78 in SGC7901-H78 cells, the expres-sion of cyclin D1 was also elevated .CONCLUSION:GRP78 might be a key player to be involved in the growth of gastric cancer.