CAJ | 학술논문

目的：探索CDX2过表达对人胃癌SGC-7901细胞增殖、生长和细胞周期的影响及其分子机制。方法：采用携带CDX2基因的重组慢病毒颗粒（ LV-CDX2-GFP）感染SGC-7901细胞，作为实验组（ LV-CDX2-GFP组）；以对照慢病毒颗粒（ LV-GFP）感染SGC-7901细胞，作为阴性对照组（ LV-GFP组）；空白对照组常规培养，不做任何处理。分别采用CCK-8法检测细胞的增殖活力，流式细胞术检测各组细胞周期的分布，半定量逆转录-聚合酶链反应（ RT-PCR）和Western blotting 技术检测细胞中CDX2、Bax、Bcl-2、cyclin D1和survivin mRNA和蛋白的表达。结果：与LV-GFP组和空白对照组比较，LV-CDX2-GFP组细胞增殖活力明显降低（P＜0.05），G0/G1期所占比例上升（P＜0.05），Bcl-2、cyclin D1和survivin mRNA和蛋白的表达降低（P＜0.05），Bax mRNA和蛋白的表达上调（P＜0.05），而LV-GFP组与空白对照组比较，差异无统计学意义（P＞0.05）。结论：慢病毒介导的CDX2过表达抑制胃癌细胞增殖和生长，使细胞周期停滞在G0/G1期，其机制可能与CDX2过表达使胃癌细胞Bcl-2、cyclin D1、survivin表达下调和Bax表达上调有关。
목적：탐색CDX2과표체대인위암SGC-7901세포증식、생장화세포주기적영향급기분자궤제。방법：채용휴대CDX2기인적중조만병독과립（ LV-CDX2-GFP）감염SGC-7901세포，작위실험조（ LV-CDX2-GFP조）；이대조만병독과립（ LV-GFP）감염SGC-7901세포，작위음성대조조（ LV-GFP조）；공백대조조상규배양，불주임하처리。분별채용CCK-8법검측세포적증식활력，류식세포술검측각조세포주기적분포，반정량역전록-취합매련반응（ RT-PCR）화Western blotting 기술검측세포중CDX2、Bax、Bcl-2、cyclin D1화survivin mRNA화단백적표체。결과：여LV-GFP조화공백대조조비교，LV-CDX2-GFP조세포증식활력명현강저（P＜0.05），G0/G1기소점비례상승（P＜0.05），Bcl-2、cyclin D1화survivin mRNA화단백적표체강저（P＜0.05），Bax mRNA화단백적표체상조（P＜0.05），이LV-GFP조여공백대조조비교，차이무통계학의의（P＞0.05）。결론：만병독개도적CDX2과표체억제위암세포증식화생장，사세포주기정체재G0/G1기，기궤제가능여CDX2과표체사위암세포Bcl-2、cyclin D1、survivin표체하조화Bax표체상조유관。
AIM:To study the effect and the molecular mechanism of CDX 2 over-expression on the prolifera-tion, growth and cell cycle of human gastric cancer cell line SGC-7901.METHODS:The SGC-7901 cells in LV-CDX2-GFP group were transfected with the recombinant lentivirus vector LV-CDX2-GFP, the cells in LV-GFP group were trans-fected with the negative control lentiviral vector for the negative control , and the cells in blank control group were without any treatment.The cell proliferation was detected by CCK-8 assay.The cell cycle distribution was analyzed by flow cytome-try.The expression of CDX2, Bax, Bcl-2, cyclin D1 and survivin was determined by semi-quantitative RT-PCR and Wes-tern blotting .RESULTS:Compared with LV-GFP group and blank control group , the proliferation activity of the SGC-7901 cells was significantly lower (P<0.05), the G0/G1 phase proportion increased (P<0.05), the mRNA and protein levels of Bcl-2, cyclin D1 and survivin were reduced (P<0.05), and the mRNA and protein levels of Bax were up-regula-ted (P<0.05) in LV-CDX2-GFP group.No statistically significant difference of the above indexes was observed (P>0.05) between LV-GFP group and blank control group .CONCLUSION:Over-expression of CDX2 mediated by lentivirus inhibits the proliferation and growth of human gastric cancer SGC-7901 cells and arrestes the cell cycle at G 0/G1 phase, which may be related to down-regulation of Bcl-2, cyclin D1 and survivin and up-regulation of Bax .