中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2014年
3期
179-182
,共4页
小鼠%肾移植%模型
小鼠%腎移植%模型
소서%신이식%모형
Mice%Kidney transplantation%Model
目的 探讨利用同一小鼠双侧肾脏分别建立小鼠肾移植模型的手术方法.方法 采用Balb/c系雄性小鼠为肾移植的供、受鼠,通过对供鼠腹部血管的精细解剖及修整,切取左、右侧肾脏分别移植给2个小鼠,供肾动脉与受鼠腹主动脉端侧吻合,供肾静脉与受鼠下腔静脉端侧吻合,动静脉的吻合方式均采用180°双定点连续外翻吻合;尿道重建采用将供肾输尿管末端引入膀胱内加外固定的方法.术后1周行二期手术,切除受鼠的自体肾脏.结果 共切取了15只供鼠的左、右侧肾脏各15个,进行了小鼠肾移植30只.30只受鼠的总体手术成功率为76.7％(23/30),其中左肾移植为80％(12/15),右肾移植为73.3％(11/15).供鼠手术耗时(33±9)min,受鼠手术耗时(46±12)min;供肾热缺血时间约10s,同一供鼠2个供肾中先移植的供肾冷缺血时间约0.5h,后移植的供肾约1.5h.肾移植后所有受鼠的血清肌酐水平均保持平稳,左肾移植受鼠与右肾移植受鼠的血清肌酐水平及其变化相同.术后2周时病理检查结果示移植肾组织具有完好的肾实质.结论 通过精细的显微解剖与术后操作,可以同时利用小鼠的双侧肾脏来分别建立小鼠肾移植模型;在提供良好模型的同时减少了实验费用.
目的 探討利用同一小鼠雙側腎髒分彆建立小鼠腎移植模型的手術方法.方法 採用Balb/c繫雄性小鼠為腎移植的供、受鼠,通過對供鼠腹部血管的精細解剖及脩整,切取左、右側腎髒分彆移植給2箇小鼠,供腎動脈與受鼠腹主動脈耑側吻閤,供腎靜脈與受鼠下腔靜脈耑側吻閤,動靜脈的吻閤方式均採用180°雙定點連續外翻吻閤;尿道重建採用將供腎輸尿管末耑引入膀胱內加外固定的方法.術後1週行二期手術,切除受鼠的自體腎髒.結果 共切取瞭15隻供鼠的左、右側腎髒各15箇,進行瞭小鼠腎移植30隻.30隻受鼠的總體手術成功率為76.7％(23/30),其中左腎移植為80％(12/15),右腎移植為73.3％(11/15).供鼠手術耗時(33±9)min,受鼠手術耗時(46±12)min;供腎熱缺血時間約10s,同一供鼠2箇供腎中先移植的供腎冷缺血時間約0.5h,後移植的供腎約1.5h.腎移植後所有受鼠的血清肌酐水平均保持平穩,左腎移植受鼠與右腎移植受鼠的血清肌酐水平及其變化相同.術後2週時病理檢查結果示移植腎組織具有完好的腎實質.結論 通過精細的顯微解剖與術後操作,可以同時利用小鼠的雙側腎髒來分彆建立小鼠腎移植模型;在提供良好模型的同時減少瞭實驗費用.
목적 탐토이용동일소서쌍측신장분별건립소서신이식모형적수술방법.방법 채용Balb/c계웅성소서위신이식적공、수서,통과대공서복부혈관적정세해부급수정,절취좌、우측신장분별이식급2개소서,공신동맥여수서복주동맥단측문합,공신정맥여수서하강정맥단측문합,동정맥적문합방식균채용180°쌍정점련속외번문합;뇨도중건채용장공신수뇨관말단인입방광내가외고정적방법.술후1주행이기수술,절제수서적자체신장.결과 공절취료15지공서적좌、우측신장각15개,진행료소서신이식30지.30지수서적총체수술성공솔위76.7％(23/30),기중좌신이식위80％(12/15),우신이식위73.3％(11/15).공서수술모시(33±9)min,수서수술모시(46±12)min;공신열결혈시간약10s,동일공서2개공신중선이식적공신랭결혈시간약0.5h,후이식적공신약1.5h.신이식후소유수서적혈청기항수평균보지평은,좌신이식수서여우신이식수서적혈청기항수평급기변화상동.술후2주시병리검사결과시이식신조직구유완호적신실질.결론 통과정세적현미해부여술후조작,가이동시이용소서적쌍측신장래분별건립소서신이식모형;재제공량호모형적동시감소료실험비용.
Objective Both kidneys of mice used To investigate the surgical procedures for establishment of murine kidney transplantation models by using bilaterial donor kidneys as grafts for two recipients.Method Using Balb/c male mice as donors/receptors,through careful dissection and trimming of donor renal vessels and abdominal great vessels,both right and left kidneys were harvested and separately transplanted into two recipients.The anastomosis between the aorta and vena cava of the graft and recipient mouse was made in end to-side manner using the 10-0 sutures.The artery and vein was anastomosed by dual point 180 continuous sutures.The technique of pulling the end of graft ureter into recipient's bladder and external fixation method were used for urethral reconstruction.Five days later the recipient's kidney was removed after transplantation.Result The kidneys in 30 mice were transplanted (15 left kidneys and 15 right kidneys) and model survival rate was 76.7％ (12/15 for the left and 11/15 for the right).The average operating time of donor was 339 min and that of recipient was 46 12 min.Cold ischemia time for the first and second transplantation was about 0.5 h and 1.5 h respectively.Conclusion Through careful microsurgical operation we establish the mouse kidney transplantation models using both donor kidneys as grafts,which reduces the costs of microsurgery experiments.
