CAJ | 학술논문

目的：探讨高压氧（HBO）对基质金属蛋白酶-9（MMP-9）在蛛网膜下腔出血（SAH）后的表达的影响及其对血脑屏障（BBB）的保护作用。方法：采用自体股动脉血枕大池单次注血法建立大鼠实验性SAH模型。造模成功后分不同时间点对HBO组给予高压氧暴露。并分不同时间点测脑含水量、BBB通透性及MMP-9的表达情况。结果：SAH后各组脑含水量、BBB通透性、MMP-9表达较Sham组显著升高（P＜0．05），但在同一时间点HBO组较SAH组脑含水量、BBB通透性、MMP-9表达显著减少（P＜0．05）。结论：HBO可能通过抑制MMP-9的表达，减轻了BBB的破坏，从而减轻脑水肿，对大鼠SAH后BBB具有保护作用。
목적：탐토고압양（HBO）대기질금속단백매-9（MMP-9）재주망막하강출혈（SAH）후적표체적영향급기대혈뇌병장（BBB）적보호작용。방법：채용자체고동맥혈침대지단차주혈법건립대서실험성SAH모형。조모성공후분불동시간점대HBO조급여고압양폭로。병분불동시간점측뇌함수량、BBB통투성급MMP-9적표체정황。결과：SAH후각조뇌함수량、BBB통투성、MMP-9표체교Sham조현저승고（P＜0．05），단재동일시간점HBO조교SAH조뇌함수량、BBB통투성、MMP-9표체현저감소（P＜0．05）。결론：HBO가능통과억제MMP-9적표체，감경료BBB적파배，종이감경뇌수종，대대서SAH후BBB구유보호작용。
Objective :To observe the impact of hyperbaric oxygen (HBO ) therapy to the expression of Matrix Metalloproteinase-9(MMP-9)and the protective effect to the blood brain barrier (BBB) permeability after subarachnoid hemorrhage (SAH) .Methods :When SAH model was made ,the HBO group received HBO therapy at predetermined time and the brain water content ,BBB permeability and the expression of MMP-9 was measured at predetermined time .Results :the brain water content ,BBB permeability and the expression of MMP-9 in each of SAH group had got a significant increase than the Sham group (P<0 .05) ,and it’s was suppressed in HBO group than in the SAH group at the same time point (P<0 .05) .Conclusion :HBO therapy by decreasing the expression of MMP-9 ,reducing BBB permeability and cerebral edema after SAH ,protected BBB from injury .