CAJ | 학술논문

目的：探讨磷酸二酯酶-4抑制剂罗氟司特对吸烟鼠肺组织MUC5AC表达的影响及其分子机制。方法：将Wistar大鼠随机分为对照组、烟雾组、罗氟司特组、烟雾＋罗氟司特组。对照组和罗氟司特组大鼠置于无烟环境中饲养，烟雾组和烟雾＋罗氟司特组大鼠暴露于有烟环境，免疫组化法检测各组大鼠肺组织MUC5AC的表达，PCR法检测MUC5ACmRNA表达量，Western-Blot检测MUC5AC蛋白的表达情况。结果：与对照组相比，烟雾组大鼠肺组织MUC5AC的mR-NA及蛋白表达水平明显升高（P＜0．05），而烟雾＋罗氟司特组MUC5AC的表达水平较烟雾组显著降低（P＜0．05）。结论：磷酸二酯酶-4抑制剂罗氟司特通过抑制吸烟鼠肺组织MUC5AC的表达，改善COPD。
목적：탐토린산이지매-4억제제라불사특대흡연서폐조직MUC5AC표체적영향급기분자궤제。방법：장Wistar대서수궤분위대조조、연무조、라불사특조、연무＋라불사특조。대조조화라불사특조대서치우무연배경중사양，연무조화연무＋라불사특조대서폭로우유연배경，면역조화법검측각조대서폐조직MUC5AC적표체，PCR법검측MUC5ACmRNA표체량，Western-Blot검측MUC5AC단백적표체정황。결과：여대조조상비，연무조대서폐조직MUC5AC적mR-NA급단백표체수평명현승고（P＜0．05），이연무＋라불사특조MUC5AC적표체수평교연무조현저강저（P＜0．05）。결론：린산이지매-4억제제라불사특통과억제흡연서폐조직MUC5AC적표체，개선COPD。
Objective :To investigate the effect of phosphodiesterase-4 inhibitor roflumilast on M UC5AC expression in smoking rat lung tissue and its molecular mechanism .Methods :Wistar rats were randomly divided in-to control group ,smoking group ,roflumilast group ,smoking + roflumilast group .Control group and roflumilast group rats were placed in smoke-free environment ,while ,rats in smoking group and smoking with roflumilast group were exposed to smoke environment .Immunohistochemical method was used to detect the MUC5AC expression in lung tissue of each group .The MUC5AC mRNA expression was measured by RT-PCR ,the protein expression of MUC5AC was measured by Western Blot .Results :As compared with the control group ,the expression level of MUC5AC mRNA and protein were significantly increased in lung tissue of smoking group ,While ,the expression level of MUC5AC in smoking + roflumilast group was reduced significantly as compared with the smoking group . Conclusion :phosphodiesterase-4 inhibitor roflumilast can reverse the smoking induced MUC5AC expression in rat lung tissue and improve COPD .