CAJ | 학술논문

目的：研究静脉注射胚胎干细胞（ESC）和川穹嗪（ TMP）对肺纤维化大鼠的治疗作用。方法：气管内滴注博莱霉素8．5 mg/kg制作雌性大鼠的肺纤维化模型。治疗组分别单独（联合）静脉注射大鼠ESC与川穹嗪，对照组注射生理盐水。记录大鼠的生存状态，测定大鼠肺组织的羟脯氨酸含量，肺脏病理学观察炎症状态、细胞凋亡情况以及相关蛋白的变化。结果：与对照组相比，接受ESC与T M P联合治疗后，肺纤维化模型大鼠的体质量增加，状态良好；肺羟脯氨酸含量明显降低；肺脏病理检查显示肺组织炎症程度降低，结构破坏减轻。流式细胞结果显示细胞凋亡指数降低，其核心蛋白MMP-2和MMP-9的表达明显下调。结论：静脉注射ESC与TMP可以减轻博莱霉素诱导的大鼠肺部炎症和肺纤维化。
목적：연구정맥주사배태간세포（ESC）화천궁진（ TMP）대폐섬유화대서적치료작용。방법：기관내적주박래매소8．5 mg/kg제작자성대서적폐섬유화모형。치료조분별단독（연합）정맥주사대서ESC여천궁진，대조조주사생리염수。기록대서적생존상태，측정대서폐조직적간포안산함량，폐장병이학관찰염증상태、세포조망정황이급상관단백적변화。결과：여대조조상비，접수ESC여T M P연합치료후，폐섬유화모형대서적체질량증가，상태량호；폐간포안산함량명현강저；폐장병리검사현시폐조직염증정도강저，결구파배감경。류식세포결과현시세포조망지수강저，기핵심단백MMP-2화MMP-9적표체명현하조。결론：정맥주사ESC여TMP가이감경박래매소유도적대서폐부염증화폐섬유화。
Objective:To investigate the therapeutic effect of tetramethylpyrazine and embryonic stem cells in pulmonary fibrosis of rats .Methods:Intratracheal instillation of the bleomycin 8 .5 mg/kg to produce female rats model of pulmonary fibrosis .Treatment groups were injected with ESC or/and Chuanqiongqin ,compared with the control group with saline .The survival state of the rats were recorded .Meanwhile ,the content of hypdroxypro-line (Hyp) in lung tissue was assayed .Detection of the lung pathology ,cell apoptosis and the activities of apoptosis related proteins were processed .Results :Compared with the control group ,the group of ESC and TMP therapy had a good condition with the increasing of body weight ;lung hydroxyproline content was significantly reduced ;lung pathological examination showed a lower degree of lung tissue inflammation ,with the mitigation of structural dam-age .The apoptotic index was decreased by flow cytometry ,and the expression of MMP-2 and MMP-9 were signifi-cantly down-regulated .Conclusion:ESC and TMP might be a therapeutic medicine for pulmonary fibrosis .