CAJ | 학술논문

目的：由于转基因大豆及其制品日益增多，其安全性亦受到了越来越多消费者的关注。为满足消费者的知情选择权，本研究建立一种快速检测外源基因的方法。方法以转基因豆制品中存在的CaMV35S启动子和NOS终止子为检测目标，优化了二重 PCR 反应体系和条件，包括引物比例和酶的用量；运用聚丙烯酰胺凝胶电泳和琼脂糖凝胶电泳对PCR产物进行检测，并对聚丙烯凝胶电泳的银染条件进行探索研究。结果建立了运用聚丙烯酰胺凝胶电泳更好地区分出外源基因CaMV35S启动子和NOS终止子的目的条带的方法。结论本实验中的二重PCR和聚丙烯酰胺凝胶电泳方法适用于对转基因豆制品中这两个外源基因的检测，为转基因豆制品检测提供了指导。
목적：유우전기인대두급기제품일익증다，기안전성역수도료월래월다소비자적관주。위만족소비자적지정선택권，본연구건립일충쾌속검측외원기인적방법。방법이전기인두제품중존재적CaMV35S계동자화NOS종지자위검측목표，우화료이중 PCR 반응체계화조건，포괄인물비례화매적용량；운용취병희선알응효전영화경지당응효전영대PCR산물진행검측，병대취병희응효전영적은염조건진행탐색연구。결과건립료운용취병희선알응효전영경호지구분출외원기인CaMV35S계동자화NOS종지자적목적조대적방법。결론본실험중적이중PCR화취병희선알응효전영방법괄용우대전기인두제품중저량개외원기인적검측，위전기인두제품검측제공료지도。
Objective Account of the increasing amount of transgenic soybean and soybean products, the safety problem has received more and more attention from consumers. In order to satisfy consumers’ right of in-formed choice, a rapid method of detecting genetically modified products was established in this study. Methods CaMV35S promoter and NOS terminator in soybean products were taken as detection target. The double PCR reac-tion system and conditions were optimized, including primer ratio and enzyme amount. Agarose gel and polyacryla-mide gel electrophoresis were applied to analyze PCR products, and the silver staining conditions for polyacrylamide gel electrophoresis were explored. Results The polyacrylamide gel electrophoresis was established and could dis-tinguish CaMV35S promoter and NOS terminator better than agarose gel. Conclusion The results showed that double PCR and polyacrylamide gel electrophoresis in this experiment were suitable for the analytic detection of two foreign genes in transgenic soybean products, and it could be a guidance for detecting transgenic food.