CAJ | 학술논문

目的：从养殖半滑舌鳎肠道分离到一株副溶血性弧菌 Vp1107,对该菌株进行耐药性及耐药分子机制研究。方法通过 K-B 法进行药敏试验, PCR 扩增及测序法对耐药基因及整合子进行检测与分析。结果Vp1107菌株耐受氨苄西林、阿莫西林、头孢唑啉、四环素、土霉素和氯霉素,对头孢呋辛钠、链霉素、卡那霉素、萘啶酸、环丙沙星中度敏感,其多重耐药系数为0.33。菌株携带blaTEM、strA、strB、tetB、tetM、catⅢ、intI1耐药相关基因,1类整合子不含基因盒, gyrA和parC基因的喹诺酮类耐药决定区未发生点突变。结论副溶血性弧菌 Vp1107耐药程度较为严重,其耐药性主要由耐药基因编码的耐药性酶类和外排泵作用引起,提示应加强对副溶血性弧菌耐药性的监控及渔用药物的管理。
목적：종양식반활설탑장도분리도일주부용혈성호균 Vp1107,대해균주진행내약성급내약분자궤제연구。방법통과 K-B 법진행약민시험, PCR 확증급측서법대내약기인급정합자진행검측여분석。결과Vp1107균주내수안변서림、아막서림、두포서람、사배소、토매소화록매소,대두포부신납、련매소、잡나매소、내정산、배병사성중도민감,기다중내약계수위0.33。균주휴대blaTEM、strA、strB、tetB、tetM、catⅢ、intI1내약상관기인,1류정합자불함기인합, gyrA화parC기인적규낙동류내약결정구미발생점돌변。결론부용혈성호균 Vp1107내약정도교위엄중,기내약성주요유내약기인편마적내약성매류화외배빙작용인기,제시응가강대부용혈성호균내약성적감공급어용약물적관리。
Objective The drug resistance and the molecular resistance mechanism of a Vibrio parahae-molyticus strain Vp1107 from the gut of the half-smooth tongue sole were studied. Methods The drug resis-tance was performed by K-B method and the resistance genes and integrons were determined by PCR amplifi-cation and DNA sequencing. Results Vp1107 was resistant to ampicillin, amoxycillin, cephazolin, tetracyc-line, oxytetracycline and chloramphenicol, and intermediate-sensitive to cefuroxime sodium, streptomycin, ka-namycin, nalidixic acid and ciprofloxacin. The multi-drug resistance index of this strain was 0.33. blaTEM、strA、strB、tetB、tetM、catⅢ、intI1 genes were present in Vp1107 and class 1 integron was an empty integron without any gene cassettes. There were no mutants within quinolone resistance-determining region of gyrA and parC genes. Conclusion The drug resistance of Vp1107 was relatively severe which was due to inactivated or modified enzymes and efflux pumps, indicating it is necessary to strengthen the monitoring of drug resistance of V. parahaemolyticus and the management of drugs’ usage.