CAJ | 학술논문

目的：优化建立水产品中氧化三甲胺(trimethylamine-N-oxide, TMAO)离子色谱法测定的前处理方法,并对青岛地区常见水产品的氧化三甲胺含量进行测定。方法考察了三氯乙酸(TCA)法、Tris-TCA法、Tris-乙腈法三种常用的前处理方法对离子色谱测定结果的影响并进行了优化,收集了青岛常见水产中具有代表性的品种,采用非抑制性离子色谱法对氧化三甲胺含量进行了测定。结果测定了头足类、硬骨鱼类、贝类和甲壳类等21种青岛地区常见水产品中的氧化三甲胺含量。其中,阿根廷鱿鱼TMAO含量最高,为8791.89 mg/kg；硬骨鱼TMAO含量范围为350~2300 mg/kg；甲壳类除了刀额新对虾以外,含量均在1700 mg/kg之上；贝类TMAO含量均在500 mg/kg之下。结论所测海产品中,头足类、甲壳类的氧化三甲胺含量较高,贝类含量较低,大致呈现头足类>甲壳类>硬骨鱼类>贝类的趋势。
목적：우화건립수산품중양화삼갑알(trimethylamine-N-oxide, TMAO)리자색보법측정적전처리방법,병대청도지구상견수산품적양화삼갑알함량진행측정。방법고찰료삼록을산(TCA)법、Tris-TCA법、Tris-을정법삼충상용적전처리방법대리자색보측정결과적영향병진행료우화,수집료청도상견수산중구유대표성적품충,채용비억제성리자색보법대양화삼갑알함량진행료측정。결과측정료두족류、경골어류、패류화갑각류등21충청도지구상견수산품중적양화삼갑알함량。기중,아근정우어TMAO함량최고,위8791.89 mg/kg；경골어TMAO함량범위위350~2300 mg/kg；갑각류제료도액신대하이외,함량균재1700 mg/kg지상；패류TMAO함량균재500 mg/kg지하。결론소측해산품중,두족류、갑각류적양화삼갑알함량교고,패류함량교저,대치정현두족류>갑각류>경골어류>패류적추세。
Objective The pretreatment procedure for trimethlylamine-N-oxide (TMAO) detection in aquatic products by ion chromatography was established, and TMAO contents of common aquatic prod-ucts from Qingdao local market was also measured in this study. Methods In this research, three pre-treatments based on trichloroacetic acid, tris and trichloroacetic acid, tris and methyl cyanide were com-pared to investigate the effect of them on ion chromatography determination. Some of the most common aquatic products in Qingdao were collected for TMAO contents determination, which was conducted by non-inhibitory ion chromatography. Results Twenty-one aquatic products commonly seen in Qingdao were analyzed, and the test samples consisted of teleost, crustacea, shellfish and cephalopod. The content of TMAO in Illex argentinuscastellanos was shown to be the highest (8791.89 mg/kg). TMAO contents varied from 350 mg/kg to 2300 mg/kg in teleost. All crustaceans except Metapenaeus ensi contained TMAO with concents over 1700 mg/kg. All shellfishes contained TMAO with contents under 500 mg/kg. Conclusion In the twenty-one aquatic products analyzed, TMAO contents were high both in cephalo-pods and crustaceans, while lower in shellfishes. The general TMAO content trend might be concluded as cephalopods>crustaceans>teleost>shellfish.