世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2014年
1期
161-166
,共6页
李文婕%高燕%陈一龙%王毓杰%张艺
李文婕%高燕%陳一龍%王毓傑%張藝
리문첩%고연%진일룡%왕육걸%장예
藏药%翼首草%绿原酸%马钱苷%獐牙菜苷%吴茱萸苷%大花双参苷A%UFLC-PDA
藏藥%翼首草%綠原痠%馬錢苷%獐牙菜苷%吳茱萸苷%大花雙參苷A%UFLC-PDA
장약%익수초%록원산%마전감%장아채감%오수유감%대화쌍삼감A%UFLC-PDA
Tibetan medicine%P. hookeri%chlorogenic acid%loganin%sweroside%evodia rutaecarpa glycosides%triplostoside A%UFLC-PDA
目的：建立同时快速测定藏药翼首草药材中绿原酸、马钱苷、獐牙菜苷、吴茱萸苷、大花双参苷A含量的方法。方法：采用超快速液相色谱仪、Agilent Poroshell 120 SB-C18色谱柱（100 mm×4.6 mm，2.7μm），柱温30益，流速1.0 mL·min-1，进样量4μL，流动相为：乙腈-0.2%磷酸水溶液，梯度洗脱，检测波长237 nm、325 nm。结果：绿原酸、马钱苷、獐牙菜苷、吴茱萸苷、大花双参苷 A分别在8.72~218.0、1.52~38.0、2.44~61.0、29.36~734.0、3.00~75.0μg·mL-1范围内（r>0.9996，n =6）呈良好线性关系，平均加样回收率分别为99.46%、99.41%、100.14%、98.89%、99.42%，RSD 分别为0.69%、0.66%、0.60%、1.21%、0.64%（n=9）。结论：该方法简便、准确、重复性好，可同时快速测定藏药翼首草中5种化学成分的含量。
目的：建立同時快速測定藏藥翼首草藥材中綠原痠、馬錢苷、獐牙菜苷、吳茱萸苷、大花雙參苷A含量的方法。方法：採用超快速液相色譜儀、Agilent Poroshell 120 SB-C18色譜柱（100 mm×4.6 mm，2.7μm），柱溫30益，流速1.0 mL·min-1，進樣量4μL，流動相為：乙腈-0.2%燐痠水溶液，梯度洗脫，檢測波長237 nm、325 nm。結果：綠原痠、馬錢苷、獐牙菜苷、吳茱萸苷、大花雙參苷 A分彆在8.72~218.0、1.52~38.0、2.44~61.0、29.36~734.0、3.00~75.0μg·mL-1範圍內（r>0.9996，n =6）呈良好線性關繫，平均加樣迴收率分彆為99.46%、99.41%、100.14%、98.89%、99.42%，RSD 分彆為0.69%、0.66%、0.60%、1.21%、0.64%（n=9）。結論：該方法簡便、準確、重複性好，可同時快速測定藏藥翼首草中5種化學成分的含量。
목적：건립동시쾌속측정장약익수초약재중록원산、마전감、장아채감、오수유감、대화쌍삼감A함량적방법。방법：채용초쾌속액상색보의、Agilent Poroshell 120 SB-C18색보주（100 mm×4.6 mm，2.7μm），주온30익，류속1.0 mL·min-1，진양량4μL，류동상위：을정-0.2%린산수용액，제도세탈，검측파장237 nm、325 nm。결과：록원산、마전감、장아채감、오수유감、대화쌍삼감 A분별재8.72~218.0、1.52~38.0、2.44~61.0、29.36~734.0、3.00~75.0μg·mL-1범위내（r>0.9996，n =6）정량호선성관계，평균가양회수솔분별위99.46%、99.41%、100.14%、98.89%、99.42%，RSD 분별위0.69%、0.66%、0.60%、1.21%、0.64%（n=9）。결론：해방법간편、준학、중복성호，가동시쾌속측정장약익수초중5충화학성분적함량。
This study was aimed to establish an Ultra Fast Liquid Chromatography-Photo Diode Array (UFLC-PDA) method for the simultaneous determination of five chemical components, which included chlorogenic acid, loganin, sweroside, evodia rutaecarpa glycosides and triplostoside A, in Pterocephalus hookeri h eck. Agilent Poroshell 120 SB-C18 (100 mm í 4.6 mm, 2.7 μm) was adopted, with acetonitrile-0.2% phosphoric acid solution in gradient elution as the mobile phase at the flow rate of 1.0 mL·min-1. And the injection volume was 0.4 μL. The detection wavelength was set up at 237 nm and 325 nm. And the column temperature was 30℃. The results showed that the calibration curve was linear within the range of 8.72~218.0, 1.52~38.0, 2.44~61.0, 29.36~734.0, 3.00~75.0μg·mL-1 (r > 0.999 6, n=9) for chlorogenic acid, loganin, sweroside, evodia rutaecarpa glycosides and triplostoside A, respectively. The average recovery rates were 99.46%, 99.41%, 100.14%, 98.89%, and 99.42%, respectively. The RSD was 0.69%, 0.66%, 0.60%, 1.21%, and 0.64%, respectively (n = 9). It was concluded that this method was simple, accurate and reproducible, which can be used for the simultaneous determination of the content of five chemical components in P. hookeri.
