CAJ | 학술논문

目的：本文建立了HPLC同时测定藏药川西千里光中绿原酸、金丝桃苷和木犀草苷3种成分的方法。方法：采用SinoChrom ODS-BP色谱柱（250 mm×4.6 mm，5μm），以乙腈（A）-0.1%磷酸溶液（B）为流动相，梯度洗脱，流速1 mL·min-1，检测波长355 nm，进样量10μL，柱温30益。结果：绿原酸、金丝桃苷、木犀草苷在30 min内均达到基线分离，各成分在其线性范围内均呈现良好的线性关系，线性范围分别为：0.6972～5.2290μg（r =0.9994）、0.0858～0.6438μg（r =1.0000）、0.0827～0.6204μg（r=1.0000）。平均加样回收率分别为：102.09%（RSD=0.99%）、101.17%（RSD=0.69%）、100.91%（RSD=1.01%）。结论：本方法操作简便、准确，重复性和稳定性好，可用于川西千里光的质量控制。
목적：본문건립료HPLC동시측정장약천서천리광중록원산、금사도감화목서초감3충성분적방법。방법：채용SinoChrom ODS-BP색보주（250 mm×4.6 mm，5μm），이을정（A）-0.1%린산용액（B）위류동상，제도세탈，류속1 mL·min-1，검측파장355 nm，진양량10μL，주온30익。결과：록원산、금사도감、목서초감재30 min내균체도기선분리，각성분재기선성범위내균정현량호적선성관계，선성범위분별위：0.6972～5.2290μg（r =0.9994）、0.0858～0.6438μg（r =1.0000）、0.0827～0.6204μg（r=1.0000）。평균가양회수솔분별위：102.09%（RSD=0.99%）、101.17%（RSD=0.69%）、100.91%（RSD=1.01%）。결론：본방법조작간편、준학，중복성화은정성호，가용우천서천리광적질량공제。
This study was aimed to establish a high-performance liquid chromatography (HPLC) method for simulta-neous determination of chlorogenic acid, hyperoside and luteoloside in Senecio solidagineus. Samples were analyzed on SinoChrom ODS-BP (250 mm í 4.6 mm, 5 μm) with acetonitrile (A) and water containing 0.1% phosphate (B) as mobile phases for gradient elution at the flow rate of 1.0 mL·min-1. The detection wavelength was set at 355 nm. The injection volume was 10 μL. The column temperature was 30oC. The results showed that chlorogenic acid, hypero-side and luteoloside achieved baseline separation within 30 min. A good linearity was observed in the range of 0.697 2~5.229 0 μg (r = 0.999 4), 0.0858~0.6438 μg (r = 1.000 0), 0.082 7~0.620 4 μg (r = 1.000 0) for chloro-genic acid, hyperoside and luteoloside, respectively, with the average recoveries of 102.09% (RSD = 0.99%), 101.17% (RSD = 0.69%), and 100.91% (RSD = 1.01%). It was concluded that the method was simple, accurate and reproducible, which can be used for the quality control of S. solidagineus.