世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2014年
1期
146-150
,共5页
杨洋%苏永文%刘悦%游佳莉%张艺%王毓杰
楊洋%囌永文%劉悅%遊佳莉%張藝%王毓傑
양양%소영문%류열%유가리%장예%왕육걸
藏药%西藏沙棘%质量标准%显微鉴别%薄层鉴别%含量测定
藏藥%西藏沙棘%質量標準%顯微鑒彆%薄層鑒彆%含量測定
장약%서장사극%질량표준%현미감별%박층감별%함량측정
Tibetan medicine%fruit of H. tibetana%quality standards%microscopic identification%TLC%content determination
目的:建立西藏沙棘药材质量标准。方法:采用显微法及TLC法鉴别西藏沙棘药材;按照2010年版《中国药典》(一部)测定西藏沙棘药材水分、总灰分、酸不溶性灰分及醇溶性浸出物含量;采用HPLC法测定其槲皮素、山柰素及异鼠李素含量。结果:确定西藏沙棘药材显微特征,建立槲皮素、山柰素及异鼠李素3种黄酮类成分的薄层鉴别及含量测定方法。结论:本研究建立的定性、定量方法,可用于西藏沙棘药材质量控制。
目的:建立西藏沙棘藥材質量標準。方法:採用顯微法及TLC法鑒彆西藏沙棘藥材;按照2010年版《中國藥典》(一部)測定西藏沙棘藥材水分、總灰分、痠不溶性灰分及醇溶性浸齣物含量;採用HPLC法測定其槲皮素、山柰素及異鼠李素含量。結果:確定西藏沙棘藥材顯微特徵,建立槲皮素、山柰素及異鼠李素3種黃酮類成分的薄層鑒彆及含量測定方法。結論:本研究建立的定性、定量方法,可用于西藏沙棘藥材質量控製。
목적:건립서장사극약재질량표준。방법:채용현미법급TLC법감별서장사극약재;안조2010년판《중국약전》(일부)측정서장사극약재수분、총회분、산불용성회분급순용성침출물함량;채용HPLC법측정기곡피소、산내소급이서리소함량。결과:학정서장사극약재현미특정,건립곡피소、산내소급이서리소3충황동류성분적박층감별급함량측정방법。결론:본연구건립적정성、정량방법,가용우서장사극약재질량공제。
This study was aimed to establish quality standards of fruit of Hippophae tibetana Schlechtend.. The medical material was identified by microscopy and thin layer chromatography (TLC). According to methods recorded in the Chinese Pharmacopoeia (2010 edition), the contents of moisture, total ash, acid-insoluble ash and alcohol-sol-uble extract were determined. The HPLC method was applied in the determination of quercetin, kaempferol and isorhamnetin content. The results showed that the microscopic characteristics of Tibetan medicine H. tibetana were identified. The TLC and content determination methods of quercetin, kaempferol and isorhamnetin were estab-lished. It was concluded that the established qualitative and quantitative methods can be used for quality control of the fruit of H . tib e tana.