CAJ | 학술논문

目的：研究西黄丸挥发油组分对荷瘤大鼠的抗肿瘤作用及其免疫学机制，筛选西黄丸抗肿瘤的活性部位。方法：雌性Wistar大鼠70只，随机抽取10只为空白对照组，剩余60只建立Walker256乳腺癌细胞荷瘤大鼠模型，建模后随机分为阴性对照组（模型组）、挥发油高、中、低剂量组，西黄丸高剂量组和香菇多糖组（阳性对照组），每组10只，灌胃给药，2次/天，连续14天。腹主动脉采血，取瘤组织称重，计算抑瘤率；采用ELISA法检测外周血IL-2、IL-6、IL-10、IFN-γ和TGF-β水平；采用流式细胞术检测CD3+ T细胞、CD4+ T细胞、CD8+ T细胞、粘附分子B7-1（CD80）细胞含量。结果：挥发油高、中剂量组抑瘤率分别为28.4%、24.1%；与模型组比较，挥发油高、中剂量组IL-2和IFN-γ平均水平及CD3+ T细胞、CD8+ T细胞、B7-1含量均明显升高（P<0.05）。结论：西黄丸挥发油具有一定的抗肿瘤作用，是西黄丸抗肿瘤活性部位之一；西黄丸挥发油可通过上调外周血IL-2、IFN-γ水平及CD3+ T细胞、CD8+ T细胞、B7-1细胞含量提高荷瘤大鼠免疫清除功能。
목적：연구서황환휘발유조분대하류대서적항종류작용급기면역학궤제，사선서황환항종류적활성부위。방법：자성Wistar대서70지，수궤추취10지위공백대조조，잉여60지건립Walker256유선암세포하류대서모형，건모후수궤분위음성대조조（모형조）、휘발유고、중、저제량조，서황환고제량조화향고다당조（양성대조조），매조10지，관위급약，2차/천，련속14천。복주동맥채혈，취류조직칭중，계산억류솔；채용ELISA법검측외주혈IL-2、IL-6、IL-10、IFN-γ화TGF-β수평；채용류식세포술검측CD3+ T세포、CD4+ T세포、CD8+ T세포、점부분자B7-1（CD80）세포함량。결과：휘발유고、중제량조억류솔분별위28.4%、24.1%；여모형조비교，휘발유고、중제량조IL-2화IFN-γ평균수평급CD3+ T세포、CD8+ T세포、B7-1함량균명현승고（P<0.05）。결론：서황환휘발유구유일정적항종류작용，시서황환항종류활성부위지일；서황환휘발유가통과상조외주혈IL-2、IFN-γ수평급CD3+ T세포、CD8+ T세포、B7-1세포함량제고하류대서면역청제공능。
This study was aimed to explore the antitumor effect of volatile oil of X ihuang pill and its immune mecha-nism in order to screen the antitumor active site of Xihuang pill. Among 70 female Wistar rats, 10 rats were random-ly selected as the blank control group; and the other 60 rats was used in the establishment of walker 256 breast can-cer cell tumor-bearing rat models. The model rats were randomly divided into the negative control group (model group), high-dose volatile oil group, middle-dose volatile oil group, low-dose volatile oil group, high-dose Xihuang pill group, and lentinan group (positive control group), with 10 rats in each group. The intragastric administration was given twice a day for 14 days. Blood was taken from the abdominal aorta. Tumor tissues was removed and weighed to calculate the tumor inhibitory rate. ELISA method was used to detect the level of IL-2, IL-6, IL-10, IFN-γ and TGF-β in peripheral blood. The flow cytometry was used to detect the content of CD3+ T cell, CD4+ T cell, CD8+ T cell, and B7-1 cell (CD80). The results showed that the tumor inhibitory rates of volatile oil of high-dose group and middle-dose group were 28.4% and 24.1%, respectively. Compared with the model group, the average level of IL-2 and IFN-γ of volatile oil of high-dose group and middle-dose group and CD3+ T cell, CD8+ T cell, B7-1 cell con-tent were obviously increased (P< 0.05). It was concluded that volatile oil of Xihuang pill had certain antitumor ef-fect, which was one of the antitumor active sites of Xihuang pill. The volatile oil of Xihuang pill upregulates the lev-els of IL-2 and IFN-γ, as well as the contents of CD3+ T cell, CD8+ T cell, B7-1 cell in order to increase the im-mune clearance function of tumor-bearing rats.