CAJ | 학술논문

目的：研究寒水石化灰小剂的抗癌活性、免疫调节作用及抗菌活性。方法：利用血清药理学方法制备寒水石化灰小剂的含药血清，MTT法观察含药血清对体外培养的S180腹水瘤细胞和人食道癌细胞增殖率的影响及对小鼠脾淋巴细胞转化的影响，牛津杯法测定寒水石化灰小剂体外的抗菌活性。结果：寒水石化灰小剂含药血清抑制S180和人食道癌细胞增殖，对人食道癌细胞的抑制与5-氟尿嘧啶（5-Fu）有协同作用；诱导小鼠脾淋巴细胞转化率降低，但对5-Fu所致的小鼠脾细胞转化率降低有抑制作用；汤剂浓度在0.25 g·mL-1以上表现有抗菌活性。结论：体外实验表明，寒水石化灰小剂能抑制S180腹水瘤细胞和人食道癌细胞增殖，显著逆转免疫抑制剂体外诱导的小鼠脾细胞转化率降低，抗菌活性尚待进一步研究。
목적：연구한수석화회소제적항암활성、면역조절작용급항균활성。방법：이용혈청약이학방법제비한수석화회소제적함약혈청，MTT법관찰함약혈청대체외배양적S180복수류세포화인식도암세포증식솔적영향급대소서비림파세포전화적영향，우진배법측정한수석화회소제체외적항균활성。결과：한수석화회소제함약혈청억제S180화인식도암세포증식，대인식도암세포적억제여5-불뇨밀정（5-Fu）유협동작용；유도소서비림파세포전화솔강저，단대5-Fu소치적소서비세포전화솔강저유억제작용；탕제농도재0.25 g·mL-1이상표현유항균활성。결론：체외실험표명，한수석화회소제능억제S180복수류세포화인식도암세포증식，현저역전면역억제제체외유도적소서비세포전화솔강저，항균활성상대진일보연구。
This study was aimed to preliminary explore the anticancer activity, immunomodulatory effects and antimi-crobial activity of Calcitum ashing agent. Serum pharmacological method was used to prepare the serum containing Calcitum ashing agent. MTT method was used to observe the cell proliferation rate of S180 ascites tumor cells and human esophageal cancer cells cultured with the serum containing Calcitum ashing agent in vitro. In addition, this method was also used to observe the mouse spleen lymphocyte transformation rate. The Oxford Cup method was used to determine the antibacterial activity on Calcitum ashing agent in vitro. The results showed that serum containing Calcitum ashing agent inhibited the cell proliferation rate of S180 and esophageal cancer cells. Meanwhile, it had a synergistic effect with 5-fluorouracil (5-FU) on the inhibition of human esophageal cancer cells. The serum contain-ing Calcitum ashing agent induced the reduction in the mice spleen lymphocytes transformation rate. But it inhibited the reduction in the spleen lymphocytes transformation rate induced by 5-FU. The decoction with the concentration higher than 0.25 g·mL-1 had significantly inhibited the proliferation of bacteria. It was concluded that Calcitum ash-ing agent can inhibit the cell proliferation of S180 ascites tumor cells and human esophageal cancer cell in vitro. It can significantly reverse the reduction in the mice spleen lymphocytes transformation rate induced by immunode-pressant. However, the antibacterial activity still requires further study.