CAJ | 학술논문

目的：探讨纳米雄黄对皮肤鳞癌A431细胞p53、Bcl-2表达的影响及相关机制。方法通过细胞培养，应用逆转录-聚合酶链反应（ RT-PCR）技术、免疫组化法观察纳米雄黄干预p53、Bcl-2在人皮肤鳞癌A431细胞中的表达情况。结果各浓度纳米雄黄干预组、注射用顺铂（ DDP）干预组及联合用药组的Bcl-2阳性率均明显低于对照组（ P＜0．05）；随着纳米雄黄浓度的升高， Bcl -2阳性率也明显降低（ P＜0．05）；联合用药组的Bcl-2阳性率明显低于各浓度纳米雄黄干预组和DDP干预组（P＜0．05）。各浓度纳米雄黄干预组、DDP干预组及联合用药组的p53的相对灰度值（RATIO）均明显高于对照组（P＜0．05）；随着纳米雄黄浓度的升高，p53的RATIO明显升高（P＜0．05）；联合用药组的p53的RATIO明显高于各浓度纳米雄黄干预组和DDP干预组（P＜0．05）。各浓度纳米雄黄干预组条带灰度高于对照组；5％、10％、20％纳米雄黄干预组条带亮度依次变大；联合用药组条带亮度较DDP干预组、各浓度纳米雄黄干预组大，内参β-actin见不到肉眼可见的浓度变化。结论纳米雄黄可上调p53表达和下调Bcl-2表达，与DDP联合应用有协同作用，可能是纳米雄黄诱导人皮肤鳞癌A431细胞凋亡的机制之一。
목적：탐토납미웅황대피부린암A431세포p53、Bcl-2표체적영향급상관궤제。방법통과세포배양，응용역전록-취합매련반응（ RT-PCR）기술、면역조화법관찰납미웅황간예p53、Bcl-2재인피부린암A431세포중적표체정황。결과각농도납미웅황간예조、주사용순박（ DDP）간예조급연합용약조적Bcl-2양성솔균명현저우대조조（ P＜0．05）；수착납미웅황농도적승고， Bcl -2양성솔야명현강저（ P＜0．05）；연합용약조적Bcl-2양성솔명현저우각농도납미웅황간예조화DDP간예조（P＜0．05）。각농도납미웅황간예조、DDP간예조급연합용약조적p53적상대회도치（RATIO）균명현고우대조조（P＜0．05）；수착납미웅황농도적승고，p53적RATIO명현승고（P＜0．05）；연합용약조적p53적RATIO명현고우각농도납미웅황간예조화DDP간예조（P＜0．05）。각농도납미웅황간예조조대회도고우대조조；5％、10％、20％납미웅황간예조조대량도의차변대；연합용약조조대량도교DDP간예조、각농도납미웅황간예조대，내삼β-actin견불도육안가견적농도변화。결론납미웅황가상조p53표체화하조Bcl-2표체，여DDP연합응용유협동작용，가능시납미웅황유도인피부린암A431세포조망적궤제지일。
Objective To investigate the effect and mechanism of realgar nano on the expression of P 53 and Bcl-2 in skin squamous cell carcinoma A 431 cells.Methods The effect of realgar nano on the expression of P 53 and Bcl-2 in skin squamous cell carcinoma A 431 cells were observed by cell culture ,RT-PCR and immunohisto-chemistry.Results Positive rate of Bcl-2 in different concentration groups of realgar nano ,DDP group and combi-nation drug therapy group was higher than that in control group (P<0.05).Positive rate of Bcl -2 was decreased with the increase of realgar nano concentration (P<0.05).Positive rate of Bcl-2 in combination drug therapy group was lower than that in different concentration groups of realgar nano and DDP group (P<0.05).The mean gray value of p53 in different concentration groups of realgar nano ,DDP group and combination drug therapy group was higher than that in control group(P<0.05).The mean gray value of p53 was increased with the increase of realgar nano concen-tration(P<0.05).The mean gray value of p53 in combination drug therapy group was higher than that in different concentration groups of realgar nano and DDP group (P<0.05).Striped gray in different concentration groups of real-gar nano was higher than that in control group (P<0.05).Striped gray in 5%,10%and 20%realgar nano group was increased.Striped gray in combination drug therapy group was higher than that in different concentration groups of re -algar nano and DDP group(P<0.05).Conclusion Realgar nano may down regulate the expression of Bcl -2 and has synergistic effect by combination of DDP ,one of the mechanisms may be nano realgar -induced human skin squa-mous cell apoptosis of A431 cells.