风湿病与关节炎
風濕病與關節炎
풍습병여관절염
Rheumatism and Arthritis
2014年
1期
19-24
,共6页
骨关节炎%祛风散寒除湿法%TGF-β%IL-1β%Fstl-1%TIMP-3%正清风痛宁缓释片%软骨细胞%小鼠
骨關節炎%祛風散寒除濕法%TGF-β%IL-1β%Fstl-1%TIMP-3%正清風痛寧緩釋片%軟骨細胞%小鼠
골관절염%거풍산한제습법%TGF-β%IL-1β%Fstl-1%TIMP-3%정청풍통저완석편%연골세포%소서
osteoarthritis%the method of expelling wind,removing cold and eliminating dampness%TGF-β%IL-1β%fstl-1%TIMP-3%zhengqing Fengtongning%sustained-release tablets%cartilage cells%mice
目的:探讨祛风散寒除湿法治疗骨关节炎的分子机制。方法:以正清风痛宁缓释片为研究载体,通过实时定量PCR试验,检测软骨细胞转化生长因子-β、白细胞介素-1β、卵泡抑素样蛋白-1、基质金属蛋白酶组织抑制因子-3 mRNA表达水平。结果:在正清风痛宁缓释片水提液高、中、低剂量组(以下简称S100、S50、S25组)以及模型对照组软骨细胞都有软骨细胞转化生长因子-β、白细胞介素-1β、卵泡抑素样蛋白-1、基质金属蛋白酶组织抑制因子-3 mRNA表达。软骨细胞转化生长因子-βmRNA表达量,S100、S50、S25组明显高于模型对照组(P<0.01),而S25组与模型对照组比较,差异无统计学意义(P>0.05);白细胞介素-1βmRNA表达量S50组明显低于模型对照组,差异有统计学意义(P<0.01),S100组与模型对照组比较,差异有统计学意义(P<0.05);S25组白细胞介素-1β表达量与模型对照组比较,差异无统计学意义(P>0.05);卵泡抑素样蛋白-1 mRNA表达量S100、S50、S25组均高于模型对照组,但差异无统计学意义(P>0.05);基质金属蛋白酶组织抑制因子-3 mRNA表达量,S50、S100组明显高于模型对照组(P<0.01),而S25组与模型对照组比较,差异无统计学意义(P>0.05)。结论:祛风散寒除湿中药正清风痛宁缓释片对关节软骨具有保护作用,其对骨关节炎软骨的保护作用可能是通过上调软骨细胞转化生长因子-β、卵泡抑素样蛋白-1及基质金属蛋白酶组织抑制因子-3 mRNA在软骨细胞的表达及下调炎性致病因子白细胞介素-1β的表达来实现的。
目的:探討祛風散寒除濕法治療骨關節炎的分子機製。方法:以正清風痛寧緩釋片為研究載體,通過實時定量PCR試驗,檢測軟骨細胞轉化生長因子-β、白細胞介素-1β、卵泡抑素樣蛋白-1、基質金屬蛋白酶組織抑製因子-3 mRNA錶達水平。結果:在正清風痛寧緩釋片水提液高、中、低劑量組(以下簡稱S100、S50、S25組)以及模型對照組軟骨細胞都有軟骨細胞轉化生長因子-β、白細胞介素-1β、卵泡抑素樣蛋白-1、基質金屬蛋白酶組織抑製因子-3 mRNA錶達。軟骨細胞轉化生長因子-βmRNA錶達量,S100、S50、S25組明顯高于模型對照組(P<0.01),而S25組與模型對照組比較,差異無統計學意義(P>0.05);白細胞介素-1βmRNA錶達量S50組明顯低于模型對照組,差異有統計學意義(P<0.01),S100組與模型對照組比較,差異有統計學意義(P<0.05);S25組白細胞介素-1β錶達量與模型對照組比較,差異無統計學意義(P>0.05);卵泡抑素樣蛋白-1 mRNA錶達量S100、S50、S25組均高于模型對照組,但差異無統計學意義(P>0.05);基質金屬蛋白酶組織抑製因子-3 mRNA錶達量,S50、S100組明顯高于模型對照組(P<0.01),而S25組與模型對照組比較,差異無統計學意義(P>0.05)。結論:祛風散寒除濕中藥正清風痛寧緩釋片對關節軟骨具有保護作用,其對骨關節炎軟骨的保護作用可能是通過上調軟骨細胞轉化生長因子-β、卵泡抑素樣蛋白-1及基質金屬蛋白酶組織抑製因子-3 mRNA在軟骨細胞的錶達及下調炎性緻病因子白細胞介素-1β的錶達來實現的。
목적:탐토거풍산한제습법치료골관절염적분자궤제。방법:이정청풍통저완석편위연구재체,통과실시정량PCR시험,검측연골세포전화생장인자-β、백세포개소-1β、란포억소양단백-1、기질금속단백매조직억제인자-3 mRNA표체수평。결과:재정청풍통저완석편수제액고、중、저제량조(이하간칭S100、S50、S25조)이급모형대조조연골세포도유연골세포전화생장인자-β、백세포개소-1β、란포억소양단백-1、기질금속단백매조직억제인자-3 mRNA표체。연골세포전화생장인자-βmRNA표체량,S100、S50、S25조명현고우모형대조조(P<0.01),이S25조여모형대조조비교,차이무통계학의의(P>0.05);백세포개소-1βmRNA표체량S50조명현저우모형대조조,차이유통계학의의(P<0.01),S100조여모형대조조비교,차이유통계학의의(P<0.05);S25조백세포개소-1β표체량여모형대조조비교,차이무통계학의의(P>0.05);란포억소양단백-1 mRNA표체량S100、S50、S25조균고우모형대조조,단차이무통계학의의(P>0.05);기질금속단백매조직억제인자-3 mRNA표체량,S50、S100조명현고우모형대조조(P<0.01),이S25조여모형대조조비교,차이무통계학의의(P>0.05)。결론:거풍산한제습중약정청풍통저완석편대관절연골구유보호작용,기대골관절염연골적보호작용가능시통과상조연골세포전화생장인자-β、란포억소양단백-1급기질금속단백매조직억제인자-3 mRNA재연골세포적표체급하조염성치병인자백세포개소-1β적표체래실현적。
Objective:To investigate the molecular mechanism of the method of expelling wind,removing cold and eliminating dampness in the treatment of osteoarthritis.Methods:Zhengqing Fengtongning sustained-release tablets as the carrier was used,by real-time quantitative PCR assay,to detect the mRNA expression levels of TGF-β,IL-1β,Fstl-1 and TIMP-3.Results:There were mRNA expressions of TGF-β,IL-1β,Fstl-1 and TIMP-3 in the chondrocytes of the Zhengqing Fengtongning sustained-release tablets as high,medium and low dose groups (hereinafter referred to as S100,S50,S25 group)and the model control group.The mRNA expressions of TGF-β in the S100,S50,S25 groups were significantly higher than that of the model control group (P<0.01). While compared mRNA expression of TGF-β in the S25 group with that of the model control group,the difference is not signiifcant (P>0.05);the mRNA expression of IL-1β in the S50 group was signiifcantly lower than that in the model control group,the difference had statistical signiifcanc(P<0.01),comparing S100 group with the model control group,the difference was statistically signiifcant (P<0.05),Comparing IL-1β of the S25 group was no significant difference (P > 0.05);the mRNA expressions of Fstl-1 of the S25,middle and high dose groups were higher than that of the model control group,but the difference was not statistically significant (P>0.05);the mRNA expressions of TIMP-3 of the S100,S50,S25 groups were signiifcantly higher than that of the model control group (P<0.01),while comparing the S100,S25group with the model control group,the difference was not statistically signiifcant (P>0.05).Conclusion:This experimental study demonstrated that Chinese medicine with effects of expelling wind,removing cold and eliminating dampness had a protective effect on articular cartilage,which might realize by the expression of TGF-β,Fstl-1 and TIMP-3 in cartilage cells and down-regulating the expression of inlfammatory disease factor IL-1β.