风湿病与关节炎
風濕病與關節炎
풍습병여관절염
Rheumatism and Arthritis
2014年
1期
10-15
,共6页
李西海%梁文娜%叶蕻芝%刘发元%李会婷%郑春松%吴广文%许惠凤%陈文列%刘献祥
李西海%樑文娜%葉蕻芝%劉髮元%李會婷%鄭春鬆%吳廣文%許惠鳳%陳文列%劉獻祥
리서해%량문나%협홍지%류발원%리회정%정춘송%오엄문%허혜봉%진문렬%류헌상
骨关节炎%软骨%潮线%软骨退变%软骨下骨%相关性%大鼠
骨關節炎%軟骨%潮線%軟骨退變%軟骨下骨%相關性%大鼠
골관절염%연골%조선%연골퇴변%연골하골%상관성%대서
osteoarthritis%cartilage%tidal line%cartilage degeneration%subchondral bone%relevance%rats
目的：探讨骨关节炎软骨潮线漂移与软骨退变的相关性。方法：选取8周龄SD大鼠20只，随机分为正常组和造模组，每组10只。采用4%木瓜蛋白酶双膝关节腔注射复制骨关节炎模型，8周后取双侧膝关节常规固定、脱钙、包埋和切片，分别采用苏木精-伊红、番红O-固绿、甲苯胺蓝和Masson三色染色法，观察骨关节的组织形态学变化。结果：正常关节面光滑平整，细胞排列整齐，四层结构清晰，潮线完整呈波伏浪起的曲线，软骨下骨小梁排列规律；骨关节炎关节面粗糙变形，细胞排列紊乱，四层结构不清晰，可见多重潮线或潮线中断，软骨下骨硬化或囊变。结论：软骨潮线漂移是骨关节炎软骨退变进程中必经的病理变化。
目的：探討骨關節炎軟骨潮線漂移與軟骨退變的相關性。方法：選取8週齡SD大鼠20隻，隨機分為正常組和造模組，每組10隻。採用4%木瓜蛋白酶雙膝關節腔註射複製骨關節炎模型，8週後取雙側膝關節常規固定、脫鈣、包埋和切片，分彆採用囌木精-伊紅、番紅O-固綠、甲苯胺藍和Masson三色染色法，觀察骨關節的組織形態學變化。結果：正常關節麵光滑平整，細胞排列整齊，四層結構清晰，潮線完整呈波伏浪起的麯線，軟骨下骨小樑排列規律；骨關節炎關節麵粗糙變形，細胞排列紊亂，四層結構不清晰，可見多重潮線或潮線中斷，軟骨下骨硬化或囊變。結論：軟骨潮線漂移是骨關節炎軟骨退變進程中必經的病理變化。
목적：탐토골관절염연골조선표이여연골퇴변적상관성。방법：선취8주령SD대서20지，수궤분위정상조화조모조，매조10지。채용4%목과단백매쌍슬관절강주사복제골관절염모형，8주후취쌍측슬관절상규고정、탈개、포매화절편，분별채용소목정-이홍、번홍O-고록、갑분알람화Masson삼색염색법，관찰골관절적조직형태학변화。결과：정상관절면광활평정，세포배렬정제，사층결구청석，조선완정정파복랑기적곡선，연골하골소량배렬규률；골관절염관절면조조변형，세포배렬문란，사층결구불청석，가견다중조선혹조선중단，연골하골경화혹낭변。결론：연골조선표이시골관절염연골퇴변진정중필경적병리변화。
Objective:To investigate the correlation between cartilage tidal line drift and degeneration in osteoarthritis.Methods:20 SD rats of 8 weeks old were randomly and equally divided into the normal group and the model group.Injections of 4% papain were given to their double knee joint cavities to replicate the osteoarthritis models.After 8 weeks,the bilateral knee joints of the rats were taken conventionally,ifxed,decalcif ied,embedded and sliced,respectively using hematoxylin-eosin,safranin O-fast green,toluidine blue and Masson trichrome staining,observing the osteoarticular histological changes.Results:The normal joints characterized smooth articular surface,cells arranging in neat rows,four-layer structure being clear,tidal lines being complete wavy and subchondral bone trabeculas being regular; but the joints with osteoarthritis characterized deformed and rough articular surface,cells arranging in disorder,the four-layer structure being not clear,multiple tidal lines or interrupted tide lines being visible and subchondral bone trabeculas being sclerotic and cystic. Conclusion:Cartilage tidal line drift is the necessary pathological changes in the process of cartilage degeneration in osteoarthritis.
