CAJ | 학술논문

目的：研究沙利度胺对免疫性肝损伤小鼠的影响。方法采用卡介苗联合脂多糖诱导法建立免疫性肝损伤的小鼠模型后，随机分为5组：正常对照组、模型组、阳性药物组（接种后第1、3、5天ip环磷酰胺100 mg/kg）、沙利度胺（低、中、高剂量）组，连续给药15 d ，进行肝、脾脏器指数的测定；分光光度法测定血清丙氨酸氨基转移酶（ALT ）、天冬氨酸氨基转移酶（AST ）浓度以及肝匀浆丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）的含量；苏木精-伊红（HE）染色法作肝组织病理学检查、肝损伤病理分级和评分；采用ELISA法测定肝匀浆转化生长因子（TGF-β1）、IL-6的表达；RT-PCR法检测肝脏组织中肿瘤坏死因子-αmRNA 表达。结果沙利度胺各剂量组都能降低免疫性肝损伤小鼠 ALT、AST、MDA 的含量，升高SOD、GSH-Px活性，显著降低免疫性肝损伤小鼠肝脏、脾脏指数（P＜0．05或 P＜0．01）；降低免疫性肝损伤小鼠TGF-β1、IL-6的表达；抑制TNF-αmRNA表达量，减轻肝组织病理损伤程度。结论沙利度胺能缓解免疫性肝损伤小鼠肝纤维化的程度，对小鼠免疫性肝损伤起保护作用，其机制可能与抑制TGF-β1、TNF-αmRNA的表达及平衡细胞因子有关。
목적：연구사리도알대면역성간손상소서적영향。방법채용잡개묘연합지다당유도법건립면역성간손상적소서모형후，수궤분위5조：정상대조조、모형조、양성약물조（접충후제1、3、5천ip배린선알100 mg/kg）、사리도알（저、중、고제량）조，련속급약15 d ，진행간、비장기지수적측정；분광광도법측정혈청병안산안기전이매（ALT ）、천동안산안기전이매（AST ）농도이급간균장병이철（MDA）、초양화물기화매（SOD）、곡광감태과양화물매（GSH-Px）적함량；소목정-이홍（HE）염색법작간조직병이학검사、간손상병리분급화평분；채용ELISA법측정간균장전화생장인자（TGF-β1）、IL-6적표체；RT-PCR법검측간장조직중종류배사인자-αmRNA 표체。결과사리도알각제량조도능강저면역성간손상소서 ALT、AST、MDA 적함량，승고SOD、GSH-Px활성，현저강저면역성간손상소서간장、비장지수（P＜0．05혹 P＜0．01）；강저면역성간손상소서TGF-β1、IL-6적표체；억제TNF-αmRNA표체량，감경간조직병리손상정도。결론사리도알능완해면역성간손상소서간섬유화적정도，대소서면역성간손상기보호작용，기궤제가능여억제TGF-β1、TNF-αmRNA적표체급평형세포인자유관。
Objective To study the impact of thalidomide on the immunological liver injury animal model in mice .Methods The immunological liver injury animal model was established in mice by injection of Bacillus Calmette-Guerin(BCG) and lipopolysaccha-ride (LPS) .These mice were randomly divided into five groups :normal control group ,model group ,positive medicine group (cyclo-phosphamide 100 mg/kg ,ip ,1 ,3 and 5 days after inoculation) ,Salle Lenalidomide (low ,medium and high dose group) ,and all of the mice were continuously administrated for 15 days ,the index of liver and spleen were detected ;Spectrophotometric was used to detect the levels of serum ALT ,AST and the content of MDA ,SOD ,GSH-Px in liver homogenates ;HE stain was used to exanimate the liver histopathology for the pathological grading and scoring of liver injury ;ELISA was used to measure the expression of TGF-β1 , IL-6 in liver homogenates ;RT-PCR was used to detect the mRNA expression of TNF-αin liver tissue .Results Thalidomide can re-duce the ALT ,AST ,MDA contents in these mice model ,increase the SOD ,GSH-Px activities and significantly reduce the index of liver and spleen of the mice with immunological liver injury (P<0 .05 or P<0 .01);also it can reduce the TGF-β1 ,IL 6 expression , inhibit TNF-αmRNA expression ,and reduce the damage degree of liver pathology .Conclusion Thalidomide can relieve the liver fi-brosis after immunological liver injury ,playing a protective role in the immunological liver injury mice model ,and its mechanism may be related to the inhibition of TGF-β1 and TNF-αexpression and the balance of cytokines .