CAJ | 학술논문

目的：探讨转录因子叉头框蛋白O3a（FOXO3a）与基质金属蛋白酶9（MMP-9）在结直肠癌组织中的表达以及二者的关系。方法应用组织芯片和免疫组织化学SP法检测FOXO3a与MMP-9在78例结直肠癌组织和69例相应正常肠黏膜组织中的表达，分析FOXO3a和MMP-9蛋白表达与结直肠癌患者临床病理特征的关系及FOXO3a和MMP-9的关系。结果（1）78例结直肠癌组织与69例正常肠黏膜组织中，FOXO3a的阳性表达率分别为57．69％（45/78）和95．65％（66/69），MMP-9的阳性表达率分别为85．90％（67/78）和15．94％（11/69），FOXO3a与MMP-9在结直肠癌组织和正常肠黏膜组织中表达的差异均具有统计学意义（P＝0．000）。（2）结直肠癌组织中FOXO3a低表达、MMP-9过表达与肿瘤浸润深度、分化程度、淋巴结转移和TNM分期密切相关（P＜0．05），与患者年龄、性别、肿瘤大小均无关（P＞0．05）。（3）FOXO3a的表达与MMP-9的表达呈负相关（r＝-0．272，P＜0．05）。结论FOXO3a表达降低及MMP-9表达增高可能与结直肠癌的进展有密切关系，联合检测FOXO3a与MMP-9的表达水平可能对预测结直肠癌的恶性程度和预后有重要意义。
목적：탐토전록인자차두광단백O3a（FOXO3a）여기질금속단백매9（MMP-9）재결직장암조직중적표체이급이자적관계。방법응용조직심편화면역조직화학SP법검측FOXO3a여MMP-9재78례결직장암조직화69례상응정상장점막조직중적표체，분석FOXO3a화MMP-9단백표체여결직장암환자림상병리특정적관계급FOXO3a화MMP-9적관계。결과（1）78례결직장암조직여69례정상장점막조직중，FOXO3a적양성표체솔분별위57．69％（45/78）화95．65％（66/69），MMP-9적양성표체솔분별위85．90％（67/78）화15．94％（11/69），FOXO3a여MMP-9재결직장암조직화정상장점막조직중표체적차이균구유통계학의의（P＝0．000）。（2）결직장암조직중FOXO3a저표체、MMP-9과표체여종류침윤심도、분화정도、림파결전이화TNM분기밀절상관（P＜0．05），여환자년령、성별、종류대소균무관（P＞0．05）。（3）FOXO3a적표체여MMP-9적표체정부상관（r＝-0．272，P＜0．05）。결론FOXO3a표체강저급MMP-9표체증고가능여결직장암적진전유밀절관계，연합검측FOXO3a여MMP-9적표체수평가능대예측결직장암적악성정도화예후유중요의의。
Objective To investigate the expression of FOXO3a and MMP 9 in colorectal cancer tissues and their relationship . Methods Tissue microarray and immunohistochemical SP methods were used to detect the expression of FOXO 3a and MMP 9 in 78 cases of colorectal cancer tissues and 69 cases of corresponding normal intestinal mucosa tissues ,then analyze the relation be-tween their expression and the clinicopathologic parameters of the patients ,and the relation between the expression of FOXO3a and MMP 9 .Results (1)The positive expression rate of FOXO3a in 78 cases of colorectal cancer tissues and 69 cases of normal intesti-nal mucosa tissues are 57 .69% (45/78)and 95 .65% (66/69) ,the positive expression rate of MMP 9 in 78 cases of colorectal cancer tissues and 69 cases of normal intestinal mucosa tissues are 85 .90% (67/78) and 15 .94% (11/69) ,and the difference is significant (P=0 .000) .(2)The decreased expression of FOXO3a and the increased expression of MMP 9 in colorectal cancer are significantly correlated with the depth of tumor invasion ,tumor histological differentiation ,metastasis of lymph node and TNM stage (P<0 .05) ,but there is no significant relationship with patients′age ,gender and tumor size (P>0 .05) .(3)There was a negative corre-lation between the expression of FOXO3a and MMP 9 (r=0 .272 ,P<0 .05) .Conclusion The decreased expression of FOXO3a and the increased expression of MMP 9 may be closely related with carcinogenesis ,invasion and metastasis of colorectal cancer ,and their combination may be an important indicator to evaluate the malignant degree and prognosis of the colorectal cancer .