CAJ | 학술논문

目的：探讨一段位于人乳头状瘤病毒（HPV）L1C-末端、长30个氨基酸残基的保守序列的免疫学性质而进行此实验。方法以此序列为基础人工合成多肽加氢氧化铝佐剂免疫小鼠。取免疫鼠脾脏制备脾细胞悬液，流式细胞术检测小鼠脾脏中CD3＋、CD3＋CD4＋、CD3＋CD8＋淋巴细胞数量，计算CD4＋/CD8＋值。四甲基偶氮唑盐（MTT）法检测细胞增殖。取培养上清液以双抗体夹心法ELISA检测细胞因子IL-4、IFN-γ的含量。结果（1）脾细胞CD3＋CD4＋细胞数量、CD4＋/CD8＋值比阴性对照组明显升高，差异有统计学意义（P＜0．05）；（2）T细胞增殖实验结果未见明显差异（P＞0．05）；（3）细胞因子IL-4含量较对照组显著增高，差异有统计学意义（P＜0．01）。IFN-γ含量与对照组比较无明显差异（P＞0．05）。结论结果表明多肽诱导体液免疫效果是肯定的，有作为预防性疫苗进一步研究开发的前景；多肽的细胞免疫原性可能较弱，未能诱导出与细胞免疫相关的反应，多肽的免疫原性还有待于进一步改进提高。
목적：탐토일단위우인유두상류병독（HPV）L1C-말단、장30개안기산잔기적보수서렬적면역학성질이진행차실험。방법이차서렬위기출인공합성다태가경양화려좌제면역소서。취면역서비장제비비세포현액，류식세포술검측소서비장중CD3＋、CD3＋CD4＋、CD3＋CD8＋림파세포수량，계산CD4＋/CD8＋치。사갑기우담서염（MTT）법검측세포증식。취배양상청액이쌍항체협심법ELISA검측세포인자IL-4、IFN-γ적함량。결과（1）비세포CD3＋CD4＋세포수량、CD4＋/CD8＋치비음성대조조명현승고，차이유통계학의의（P＜0．05）；（2）T세포증식실험결과미견명현차이（P＞0．05）；（3）세포인자IL-4함량교대조조현저증고，차이유통계학의의（P＜0．01）。IFN-γ함량여대조조비교무명현차이（P＞0．05）。결론결과표명다태유도체액면역효과시긍정적，유작위예방성역묘진일보연구개발적전경；다태적세포면역원성가능교약，미능유도출여세포면역상관적반응，다태적면역원성환유대우진일보개진제고。
Objective To investigate and detect the immunity characteristics of a conserved sequence including 30 amino residues which is located in the C terminal of HPVL1 .Methods The immune model of mouse was establish with a polypeptide synthetized based on this sequence .The amount of CD3+ ,CD3+CD4+ or CD3+CD8+ lymphocyte of the mouse spleen was detected by Flow cy-tometry ,then the value of CD4+ /CD8+ were calculated .The cell proliferation was detected by MTT assay .Sample was took from the cell supernatant to detect the content of IL-4 and IFN-γby double antibody sandwich ELISA .Results (1)The amount of CD3+CD8+ lymphocytes and the value of CD4+ /CD8+ were significantly increased than control group(P<0 .05) .(2)The result of T cell proliferation showed no significant difference (P>0 .05) .(3)The level of IL 4 were significantly higher than that of control group (P<0 .01) .The content of IFN-γlevel showed no significant difference compared with control group (P>0 .05) .Conclusion The results showed that it was affirmative that the polypeptides induced humoral immunity ,which was worth to be furtherly studied as a preventive vaccine .But its cell immunogenicity is weak ,and the attenmpt to induce the response related to the cell immunogenicity was failed ,and the immunogenicity of the peptide remains to be improved .