CAJ | 학술논문

目的：采用反相高效液相色谱法测定格列吡嗪微球中的含量。方法采用 Agilent Eclipse XDB-C18色谱柱（150 mm ×4．6 mm ，5μm），以0．1 mol/L磷酸二氢钠（用氢氧化钠溶液调节pH至6．00±0．05）-甲醇（50∶50）为流动相；流速为1．0 mL/min ，紫外检测波长为275 nm。结果格列吡嗪在5～50μg/mL与峰面积呈良好的线性关系（ r ＝0．9998），方法平均回收率为100．06％，RSD ＝0．214％。结论反相高效液相色谱法测定格列吡嗪缓释微球的含量简便易行，专属性强，灵敏度高，重复性好，结果准确。
목적：채용반상고효액상색보법측정격렬필진미구중적함량。방법채용 Agilent Eclipse XDB-C18색보주（150 mm ×4．6 mm ，5μm），이0．1 mol/L린산이경납（용경양화납용액조절pH지6．00±0．05）-갑순（50∶50）위류동상；류속위1．0 mL/min ，자외검측파장위275 nm。결과격렬필진재5～50μg/mL여봉면적정량호적선성관계（ r ＝0．9998），방법평균회수솔위100．06％，RSD ＝0．214％。결론반상고효액상색보법측정격렬필진완석미구적함량간편역행，전속성강，령민도고，중복성호，결과준학。
Objective To determine the loading amount of glipizide sustained-release microspheres by RP-HPLC .Methods An RP-HPLC method was employed for determination using Agilent Eclipse XBD-C18 column (150 mm × 4 .6 mm ,5 μm) with a mobile phase consisted of methanol-buffer solution(0 .1mol/L sodium hydrox-ide)(50∶50) ,at a flow rate of 1 .0 mL/min and the detection wavelength of 275 nm .Results Good linearity was achieved in the range of 5~50 μg/mL( r =0 .999 8) .The recovery was 100 .06% with RSD of 0 .214% .Conclu-sion T his method appeared easy-to-use ,specific ,sensitive ,reproducible and accurate ..