海洋学报(中文版)
海洋學報(中文版)
해양학보(중문판)
ACTA OCEANOLOGICA SINICA
2014年
2期
123-129
,共7页
化感物质%旋链角毛藻 Chaetoceros curv isetus%中肋骨条藻 Skeletonema costatum
化感物質%鏇鏈角毛藻 Chaetoceros curv isetus%中肋骨條藻 Skeletonema costatum
화감물질%선련각모조 Chaetoceros curv isetus%중륵골조조 Skeletonema costatum
allelochemical%Chaetoceros curvisetus%Skeletonema costatum
在已知旋链角毛藻对中肋骨条藻具有化感作用的前提下,进行了温度、生长时期、光照、细菌等因素对旋链角毛藻化感作用的影响、化感物质的萃取及粗提物对中肋骨条藻的化感效应检验等实验,研究了旋链角毛藻化感物质的性质、降解特点,并初步测定了化感物质的结构。结果表明:旋链角毛藻化感作用与生长时期有关,指数期滤液化感作用强于衰亡期;旋链角毛藻的化感物质在50℃以下保持稳定,光和细菌均引起旋链角毛藻化感物质的降解,且细菌降解强于光降解;旋链角毛藻的乙酸乙酯萃取物具有明显的化感活性,该萃取物在255~260 nm处有特征吸收峰。综合以上结果,推测旋链角毛藻化感物质可能为分解温度在50℃以上的易被光和细菌降解的苯衍生物,从极性上看较易溶于乙酸乙酯。
在已知鏇鏈角毛藻對中肋骨條藻具有化感作用的前提下,進行瞭溫度、生長時期、光照、細菌等因素對鏇鏈角毛藻化感作用的影響、化感物質的萃取及粗提物對中肋骨條藻的化感效應檢驗等實驗,研究瞭鏇鏈角毛藻化感物質的性質、降解特點,併初步測定瞭化感物質的結構。結果錶明:鏇鏈角毛藻化感作用與生長時期有關,指數期濾液化感作用彊于衰亡期;鏇鏈角毛藻的化感物質在50℃以下保持穩定,光和細菌均引起鏇鏈角毛藻化感物質的降解,且細菌降解彊于光降解;鏇鏈角毛藻的乙痠乙酯萃取物具有明顯的化感活性,該萃取物在255~260 nm處有特徵吸收峰。綜閤以上結果,推測鏇鏈角毛藻化感物質可能為分解溫度在50℃以上的易被光和細菌降解的苯衍生物,從極性上看較易溶于乙痠乙酯。
재이지선련각모조대중륵골조조구유화감작용적전제하,진행료온도、생장시기、광조、세균등인소대선련각모조화감작용적영향、화감물질적췌취급조제물대중륵골조조적화감효응검험등실험,연구료선련각모조화감물질적성질、강해특점,병초보측정료화감물질적결구。결과표명:선련각모조화감작용여생장시기유관,지수기려액화감작용강우쇠망기;선련각모조적화감물질재50℃이하보지은정,광화세균균인기선련각모조화감물질적강해,차세균강해강우광강해;선련각모조적을산을지췌취물구유명현적화감활성,해췌취물재255~260 nm처유특정흡수봉。종합이상결과,추측선련각모조화감물질가능위분해온도재50℃이상적역피광화세균강해적분연생물,종겁성상간교역용우을산을지。
On the basis of the fact that Chaetoceros curvisetus showed allelopathic effect on Skeletonema costatum ,a series of experiments ,including algal cultures and compounds extraction ,were carried out to research the proper-ties ,influence factors ,degradation characteristics and structure of C .curvisetus allelochemical .The results indica-ted that allelopathic effect of C .curvisetus was related to the growth phase ,and the C .curvisetus filtrates in expo-nential phase caused the inhibition on the grow th of S .costatum than that of declining phase filtrates .Chaetoceros curvisetus allelochemical remain stable under 50℃ temperature and degraded as time went on attributed on light and bacteria ,and bacteria contributed more .Furthermore ,the ethyl acetate extraction of C . curvisetus filtrate showed significant allelopathic effect and the wavelength of characteristic absorption for the growth inhibitor was 255-260 nm .Consequently ,the effect ingredient was dissolved by ethyl acetate .In addition ,the allelochemical of C .curvisetus was supported to be benzene derivatives whose decomposition temperature was more than 50℃ and could be degraded by light and bacteria .