CAJ | 학술논문

为了解我国近海中度嗜盐菌系统发育多样性及产酶特性，从天津、山东、江苏、福建、海南等地近海采集样品为研究对象，分离筛选得到108株中度嗜盐菌，其中有26株至少产一种酶。通过对其形态特征、生理生化、16S rRNA 基因序列及系统进化树进行分析，将26株菌鉴定为细菌域的Halomonas、Idiomarina、Virgibacillus、Pontibacillus、Oceanobacillus、Halobacillus和 Marinilactibacillus属的菌株。它们与相应属的模式菌株16S rRNA基因序列相似性在97％～100％不等，其中有些菌株可能代表不同的分类单元。底物特异性试验表明，分离的26株中度嗜盐菌13株产蛋白酶，19株产淀粉酶，13株产酯酶，4株产纤维素酶。其中6株产3种酶，11株产2种酶。研究结果表明中度嗜盐菌具有系统发育和产酶多样性，同时蕴藏着较多新的微生物类群，为嗜盐微生物资源应用开发提供了参考。
위료해아국근해중도기염균계통발육다양성급산매특성，종천진、산동、강소、복건、해남등지근해채집양품위연구대상，분리사선득도108주중도기염균，기중유26주지소산일충매。통과대기형태특정、생리생화、16S rRNA 기인서렬급계통진화수진행분석，장26주균감정위세균역적Halomonas、Idiomarina、Virgibacillus、Pontibacillus、Oceanobacillus、Halobacillus화 Marinilactibacillus속적균주。타문여상응속적모식균주16S rRNA기인서렬상사성재97％～100％불등，기중유사균주가능대표불동적분류단원。저물특이성시험표명，분리적26주중도기염균13주산단백매，19주산정분매，13주산지매，4주산섬유소매。기중6주산3충매，11주산2충매。연구결과표명중도기염균구유계통발육화산매다양성，동시온장착교다신적미생물류군，위기염미생물자원응용개발제공료삼고。
To investigate phylogenetic and enzyme production diversity of moderately halophilic bacteria ,we got the samples from China's offshore of Tianjing ,Shandong ,Jiangsu ,Fujian and Hainan. 108 moderately halophilic strains were isolated ,26 of which could produce at least one type of enzymes. According to physiological and bio-chemical characteristics ,16S rRNA sequences and phylogenetic analysis ,the 26 moderately halophilic strains be-longed to genera Halomonas ,Idiomarina ,Virgibacillus ,Pontibacillus ,Oceanobacillus ,Halobacillus or Mari-nilactibacillus separately. The sequences had the similarity (97% -100% ) to the type strains of each genera. Some moderately halophilic bacteria might be a novel species. The substrates specific experiment indicated that there were 13 moderately halophilic strains producing protease ,19 strains producing amylase ,13 strains producing ester-ase ,4 strains producing cellulose. And there were 6 strains producing three enzymes and 11 strains producing two enzymes simultaneously. The research indicated that there was not only abundant phylogenetic and enzyme diversi-ty of moderately halophilic bacteria ,but also some unknown bacteria groups existed in China's offshore ,which provided references for reasonable development and utilization of halophilic microbial resources.