CAJ | 학술논문

为分析全国海水浴场、贝类增养殖区人类肠道腺病毒的污染状况，采用超滤浓缩方法，TaqM an探针实时定量PCR技术对其进行了定量。结果表明，全国10个海水浴场表层海水中腺病毒的阳性检出率为35％，含量为1.1×107～2.8×107 copies/L。在离岸100 m和1000 m处腺病毒的分布无显著差别（ p＞0.05）；在调查的162份贝类样品中，腺病毒的阳性检出率为11.7％，含量为2.4×104～3.1×105 copies/g。6类经济贝类中牡蛎带病毒量最高。由此可见，全国范围内海水浴场表层海水和主要增养殖区经济贝类中腺病毒污染比较普遍，尤其以山东、辽宁和浙江三省最为严重，给洗浴者和消费者健康造成潜在威胁。人类肠道腺病毒可以很好地指示粪便污染，调查其在海水浴场和经济贝类中的分布，可以帮助公众预警胃肠道病毒的暴发流行，本研究为开展全国腺病毒的环境风险评估和分子流行病学调查奠定了良好的基础。
위분석전국해수욕장、패류증양식구인류장도선병독적오염상황，채용초려농축방법，TaqM an탐침실시정량PCR기술대기진행료정량。결과표명，전국10개해수욕장표층해수중선병독적양성검출솔위35％，함량위1.1×107～2.8×107 copies/L。재리안100 m화1000 m처선병독적분포무현저차별（ p＞0.05）；재조사적162빈패류양품중，선병독적양성검출솔위11.7％，함량위2.4×104～3.1×105 copies/g。6류경제패류중모려대병독량최고。유차가견，전국범위내해수욕장표층해수화주요증양식구경제패류중선병독오염비교보편，우기이산동、료녕화절강삼성최위엄중，급세욕자화소비자건강조성잠재위협。인류장도선병독가이흔호지지시분편오염，조사기재해수욕장화경제패류중적분포，가이방조공음예경위장도병독적폭발류행，본연구위개전전국선병독적배경풍험평고화분자류행병학조사전정료량호적기출。
In order to analyze the contamination of human enteric viruses in bathing beach and economic shellfish along the primarily commercial shellfish-breeding areas in coastal cities ,we chose adenovirus as the object of this study .Vi-rus was concentrated by efficient ultra-centrifuge method ,then TaqMan real-time PCR was employed to quantify its concentration .The percentage of positive samples for adenovirus was 35% in ten bathing beaches of China .The con-centration was 1.1 × 107 -2.8 × 107 copies/L .The viral incidence in the water samples collected from 100 meters off the seashore was found not to be correlated with those from 1 000 meters out (p>0.05) .The percentage of positive samples for adenovirus was 11.7% in 162 samples ,the concentration ranged from 2.4 × 104 to 3.1 × 105 copies/g . During six chosen commercial shellfish ,Oyster apparently had the strongest ability to carry adenovirus .Hence ,the contamination of seawater and shellfish by human adenovirus was relatively common . The fecal contamination in Shandong Province ,Liaoning Province and Zhejiang Province were more severe ,indicating a potential public health threat for bathers and consumers . Human enteric adenovirus was a good indicator to present the fecal pollution . Therefore ,investigating its distribution from bathing beach and commercial shellfish may help to alert community health to enteric viruses that are in circulation in the population .This study laid a good foundation for carrying out the risk assessment and molecular epidemiological investigation of adenovirus in the w hole China .