CAJ | 학술논문

目的：探讨乙型肝炎病毒（HBV）慢性化病变患者外周血单个核细胞共刺激分子CD28、CTLA-4及其配体CD80、CD86 mRNA的表达及其意义。方法采用Taqman探针实时荧光定量PCR方法分别检测24例慢性乙型肝炎（CHB组）、24例肝硬化（LC组）、28例原发性肝癌（HCC组）和30例健康人（对照组）外周血单个核细胞的共刺激分子CD28、CTLA-4、CD86、CD80 mRNA的水平。结果与对照组相比，CD28 mRNA表达在CHB组下降显著（t＝-2．11，P＜0．05）；CTLA-4 mRNA在疾病各组表达均有不同程度的显著下降：CHB组（t＝-2．52，P＜0．05），LC组（t＝-2．11，P＜0．05），HCC组（t＝-2．56，P＜0．05）；CD86 mRNA在疾病各组表达也有不同程度的显著下降：CHB组（t＝-3．68，P＜0．01），LC组（t＝-2．99，P＜0．01）， HCC组（t＝-4．42，P＜0．01）；CD28/CTLA-4 mRNA表达在HCC组显著升高（t＝2．12，P＜0．05）；CD80/CD86 mRNA表达随着HBV的慢性化进程出现不同程度显著升高：CHB组（t＝2．10，P＜0．05），LC组（t＝2．59，P＜0．05），HCC组（t＝3．74，P＜0．01）。结论 HBV感染患者CD28/B7家族共刺激分子的表达异常与其免疫功能紊乱和慢性化病变发生、发展密切相关。
목적：탐토을형간염병독（HBV）만성화병변환자외주혈단개핵세포공자격분자CD28、CTLA-4급기배체CD80、CD86 mRNA적표체급기의의。방법채용Taqman탐침실시형광정량PCR방법분별검측24례만성을형간염（CHB조）、24례간경화（LC조）、28례원발성간암（HCC조）화30례건강인（대조조）외주혈단개핵세포적공자격분자CD28、CTLA-4、CD86、CD80 mRNA적수평。결과여대조조상비，CD28 mRNA표체재CHB조하강현저（t＝-2．11，P＜0．05）；CTLA-4 mRNA재질병각조표체균유불동정도적현저하강：CHB조（t＝-2．52，P＜0．05），LC조（t＝-2．11，P＜0．05），HCC조（t＝-2．56，P＜0．05）；CD86 mRNA재질병각조표체야유불동정도적현저하강：CHB조（t＝-3．68，P＜0．01），LC조（t＝-2．99，P＜0．01）， HCC조（t＝-4．42，P＜0．01）；CD28/CTLA-4 mRNA표체재HCC조현저승고（t＝2．12，P＜0．05）；CD80/CD86 mRNA표체수착HBV적만성화진정출현불동정도현저승고：CHB조（t＝2．10，P＜0．05），LC조（t＝2．59，P＜0．05），HCC조（t＝3．74，P＜0．01）。결론 HBV감염환자CD28/B7가족공자격분자적표체이상여기면역공능문란화만성화병변발생、발전밀절상관。
Objective To analyze and evaluate the expression and significance of costimulatory molecules CD 28 ,CTLA-4 ,CD86 , CD80 mRNA in peripheral blood mononuclear cells of the patients with HBV chronic change .Methods The levels of costimulatory molecules CD28 ,CTLA-4 ,CD86 ,CD80 mRNA in peripheral blood mononuclear cells were detected in 24 cases of chronic hepatitis B (CHB) ,24 cases of liver cirrhosis(LC) ,28 cases of hepatocellular cancer(HCC) and 30 normal control(NC) subjects by real time quantitative PCR .Results Compared with the NC group ,costimulatory molecule CD28 mRNA level in the CHB group was signifi-cantly decreased(t= -2 .11 ,P<0 .05);CTLA-4 mRNA level in different diseases groups was decreased to different degrees :the CHB group(t= -2 .52 ,P<0 .05) ,the LC group(t= -2 .11 ,P<0 .05) and the HCC group(t= -2 .56 ,P<0 .05);CD86 mRNA level in different diseases groups was decreased to different degrees too :the CHB group(t= -3 .68 ,P<0 .01) ,the LC group(t= -2 .99 ,P<0 .01) and the HCC group(t= -4 .42 ,P< 0 .01);CD28/CTLA-4 mRNA level was significantly increased in the HCC group(t= 2 .12 ,P< 0 .05);CD80/CD86 mRNA level was significantly increased to different degrees with the progress of HBV chronic change:the CHB group(t=2 .10 ,P<0 .05) ,the LC group(t=2 .59 ,P<0 .05) and the HCC group(t=3 .74 ,P<0 .01) . Conclusion The expression abnormality of CD28/B7 family costimulatory molecules mRNA in HBV infectious patients may be closely related with the immune dysfunction and the development and progression of the chronic change .