CAJ | 학술논문

目的：检测HER2基因在临床结肠癌组织中的表达与患者临床病理特征的关联，同时分析其对结肠癌细胞增殖及细胞周期的影响。方法收集结肠癌患者癌组织及对应的癌旁组织108例，应用原位杂交和荧光定量PCR技术检测结肠癌组织及对应癌旁组织中HER2基因的表达水平，分析HER2基因表达与患者临床病理特征之间的关系。降低SW480和LoVo结肠癌细胞中HER2的表达，运用MTT比色法检测结肠癌细胞增殖情况，利用流式细胞技术检测结肠癌细胞周期的变化情况。结果原位杂交结果表明，在108例结肠癌组织中HER2阳性表达率为66．67％，对应癌旁组织中的阳性表达率为10．19％，两者比较差异有统计学意义（P＜0．05）；荧光定量PCR结果进一步显示，61．11％（66/108）的结肠癌组织HER2表达显著高于对应的癌旁组织（P＜0．05）；结肠癌患者的临床分期越晚，HER2基因的表达越高（P＜0．05）；另外在淋巴结转移阳性的结肠癌组织中HER2基因的表达量显著高于淋巴结转移阴性者的结肠癌组织（P＜0．05）；siRNA-HER2可以明显降低SW480和LoVo中HER2基因的表达；降低SW480和LoVo细胞中HER2的表达，其生长受到明显抑制，G0/G1期细胞比例明显增加，而S期和G2/M期细胞比例下降。结论HER2基因与结肠癌的发生发展密切相关，其机制可能为调节细胞周期中G1/S期的转换，为结肠癌患者的治疗提供了新靶点。
목적：검측HER2기인재림상결장암조직중적표체여환자림상병리특정적관련，동시분석기대결장암세포증식급세포주기적영향。방법수집결장암환자암조직급대응적암방조직108례，응용원위잡교화형광정량PCR기술검측결장암조직급대응암방조직중HER2기인적표체수평，분석HER2기인표체여환자림상병리특정지간적관계。강저SW480화LoVo결장암세포중HER2적표체，운용MTT비색법검측결장암세포증식정황，이용류식세포기술검측결장암세포주기적변화정황。결과원위잡교결과표명，재108례결장암조직중HER2양성표체솔위66．67％，대응암방조직중적양성표체솔위10．19％，량자비교차이유통계학의의（P＜0．05）；형광정량PCR결과진일보현시，61．11％（66/108）적결장암조직HER2표체현저고우대응적암방조직（P＜0．05）；결장암환자적림상분기월만，HER2기인적표체월고（P＜0．05）；령외재림파결전이양성적결장암조직중HER2기인적표체량현저고우림파결전이음성자적결장암조직（P＜0．05）；siRNA-HER2가이명현강저SW480화LoVo중HER2기인적표체；강저SW480화LoVo세포중HER2적표체，기생장수도명현억제，G0/G1기세포비례명현증가，이S기화G2/M기세포비례하강。결론HER2기인여결장암적발생발전밀절상관，기궤제가능위조절세포주기중G1/S기적전환，위결장암환자적치료제공료신파점。
Objective To detect the expression of HER2 in clinical colon carcinoma tissue ,to investigate its correlation with the clinicopathological characteristics and to analyze its influence on the proliferation and cell cycle in colon carcinoma cell lines .Methods 108 specimens of colon carcinoma and corresponding paracancerous tissues were collected .The hybridization in situ and real-time quantitative polymerase chain reaction were used to detect the HER 2 expression in those specimens .The relationship between HER2 expression and the clinicopathologic features was analyzed .The expression of HER2 in colon carcinoma cells(SW480 and Lo-Vo) was reduced by using the antisense technology .The MTT assay and the flow cytometry were used to investigate the HER2 in-fluences on the cell proliferation and cell cycles .Results The hybridization in situ results showed that the HER2 positive expres-sion rate was 66 .67% in colon carcinoma and 10 .19% in the paracancerous tissues ,the difference between them was statistically significant(P<0 .05) .The real-time fluorescent quantitative PCR results further showed that HER2 was found to be overexpressed in 61 .11% of the colon carcinoma tissue(P<0 .05);the expression of HER2 was gradually increased with the progress of colon cancer .(P<0 .05);the expression of HER2 in the colon tissue with lymph node metastasis was also significantly higher than that without lymph node metastasis in colon carcinoma (P<0 .05);siRNA-HER2 could significantly reduce the expression of HER2 in colon cancer cell lines(SW480 and LoVo) ,the growth of colon carcinoma cell lines was also significantly inhibited and the propor-tion of cells in G0/G1 phase was increased ,while the proportion of cells in S phase and G2/M phase was decreased .Conclusion HER2 is closely related with the occurrence and development of colon carcinoma ,its mechanism could regulate the grow th of colon carcinoma cells via mediating the transition of G1/S phase ,which may provide a new target for the treatment of colon carcinoma .