CAJ | 학술논문

目的：探讨原发性肝癌中磷脂酰肌醇蛋白聚糖（Glypican3）、基质金属蛋白酶-9（MMP-9）和基质金属蛋白酶-14（MMP-14）的表达特征，关注其在肿瘤发生、发展和转移中的作用。方法本试验以102例原发性肝癌作为观察组，以80例正常肝组织作为对照组，采用免疫组织化学方法检测两组中Glypican3、MMP-9和MMP-14的表达，探讨Glypican3、MMP-9和MMP-14在不同临床病理特征中的表达差别及相关性。结果观察组中Glypican3、MMP-9和MMP-14表达的阳性率明显高于对照组，观察组中Glypican3、MMP-9和MMP-14表达的阳性率与肿瘤体积、分化程度、淋巴结转移、脉管浸润、被膜侵犯和增殖组胞核抗原的表达密切相关。相关性分析显示观察组中Glypican3和MMP-9、Glypican3和MMP-14、MMP-9和MMP-14的表达均呈正相关性（r＝0．48，P＝0．0241；r＝0．46，P＝0．0132；r＝0．43．P＝0．0313）。生存分析显示Glypican3、MMP-9和MMP-14的表达与预后相关。结论原发性肝癌组织中Glypican3、MMP-9和MMP-14高表达，在肿瘤进展中有明显的促进作用，术后检测Glypican3、MMP-9和MMP-14的表达对判断原发性肝癌的预后有一定价值。
목적：탐토원발성간암중린지선기순단백취당（Glypican3）、기질금속단백매-9（MMP-9）화기질금속단백매-14（MMP-14）적표체특정，관주기재종류발생、발전화전이중적작용。방법본시험이102례원발성간암작위관찰조，이80례정상간조직작위대조조，채용면역조직화학방법검측량조중Glypican3、MMP-9화MMP-14적표체，탐토Glypican3、MMP-9화MMP-14재불동림상병리특정중적표체차별급상관성。결과관찰조중Glypican3、MMP-9화MMP-14표체적양성솔명현고우대조조，관찰조중Glypican3、MMP-9화MMP-14표체적양성솔여종류체적、분화정도、림파결전이、맥관침윤、피막침범화증식조포핵항원적표체밀절상관。상관성분석현시관찰조중Glypican3화MMP-9、Glypican3화MMP-14、MMP-9화MMP-14적표체균정정상관성（r＝0．48，P＝0．0241；r＝0．46，P＝0．0132；r＝0．43．P＝0．0313）。생존분석현시Glypican3、MMP-9화MMP-14적표체여예후상관。결론원발성간암조직중Glypican3、MMP-9화MMP-14고표체，재종류진전중유명현적촉진작용，술후검측Glypican3、MMP-9화MMP-14적표체대판단원발성간암적예후유일정개치。
Objective To investigate the expression characteristics of Glypican3 ,MMP-9 and MMP-14 in primary hepatocellular carcinoma ,and to focus on their roles on the development ,progress and metastasis of tumor .Methods 102 cases of primary hepato-cellular carcinoma were taken as the observation group and 80 cases of normal liver tissues as the control group .The expressions of Glypican3 ,MMP-9 and MMP-14 were detected by the immunohistochemistry method and their expression difference in different clinicopathological characteristics and the correlation were investigated .Results The positive rates of Glypican3 ,MMP-9 and MMP-14 expressions in the observation group were significantly higher than those in the control group .The positive rates of Glypi-can3 ,MMP-9 and MMP-14 expressions were closely correlated with the tumor volume ,differentiation degree ,lymph node metasta-sis ,vessel infiltration ,membrane invasion and PCNA expression .The correlation analysis showed that the positive relationships were found between Glypican3 and MMP-9 ,between Glypican3 and MMP-14 and between MMP-9 and MMP-14 in the observation group(r=0 .48 ,P=0 .024 1 ;r=0 .46 ,P=0 .013 2;r=0 .43 .P=0 .031 3) .The survival analysis showed that expressions of Glypi-can3 ,MMP-9 and MMP-14 were correlated with the patient′s prognosis .Conclusion The higher-expressions of Glypican3 ,MMP-9 and MMP-14 may promote the occurrence and development of primary hepatocellular carcinoma .Detecting the postoperative expres-sions of Glypican3 ,MMP-9 and MMP-14 has certqain value to judge the prognosis in primary hepatocellular carcinoma .