中国肿瘤临床
中國腫瘤臨床
중국종류림상
CHINESE JOURNAL OF CLINICAL ONCOLOGY
2014年
3期
170-174
,共5页
苏晓路%李晓鸣%田卫华%张煦
囌曉路%李曉鳴%田衛華%張煦
소효로%리효명%전위화%장후
Aurora-A%P53%c-myc%组织芯片%大肠癌
Aurora-A%P53%c-myc%組織芯片%大腸癌
Aurora-A%P53%c-myc%조직심편%대장암
Aurora-A%P53%c-myc%tissue microarray%colorectal cancer
目的：研究中心体相关激酶Aurora-A、突变型P53（mt-P53）和c-myc在大肠癌中的表达规律，探讨其相互关系及在肿瘤发生发展中的作用。方法：应用组织芯片和免疫组织化学技术（SP法）检测Aurora-A、mt-P53和c-myc在130例大肠癌、癌旁组织和正常大肠组织中的表达，并结合临床病理学参数进行综合分析。结果：Aurora-A在正常大肠组织、癌旁组织和大肠癌中的阳性表达率分别为0，35%，69%。mt-P53分别为0，20%，57%。c-myc分别为0，37%，76%。与正常大肠组织和癌旁相比，癌组织中Aurora-A、mt-P53和c-myc的表达明显升高（P<0.01）；三者的表达与肿瘤的浸润程度有关（P<0.05），mt-P53和c-myc还与Dukes'分期和淋巴结转移关系密切（P<0.05）。在大肠癌中Aurora-A的阳性表达与mt-P53、c-myc的阳性表达呈显著正相关（分别为r=0.362，P<0.01；r=0.487，P<0.01），mt-P53和c-myc之间也存在显著正相关（r=0.242，P<0.01）。结论：Aurora-A和c-myc蛋白的过表达与P53的突变在大肠癌的发生、侵袭和转移中发挥着重要作用。综合分析Aurora-A、mt-P53和c-myc蛋白的表达对大肠癌的早期诊断及预后判断具有重要价值。
目的：研究中心體相關激酶Aurora-A、突變型P53（mt-P53）和c-myc在大腸癌中的錶達規律，探討其相互關繫及在腫瘤髮生髮展中的作用。方法：應用組織芯片和免疫組織化學技術（SP法）檢測Aurora-A、mt-P53和c-myc在130例大腸癌、癌徬組織和正常大腸組織中的錶達，併結閤臨床病理學參數進行綜閤分析。結果：Aurora-A在正常大腸組織、癌徬組織和大腸癌中的暘性錶達率分彆為0，35%，69%。mt-P53分彆為0，20%，57%。c-myc分彆為0，37%，76%。與正常大腸組織和癌徬相比，癌組織中Aurora-A、mt-P53和c-myc的錶達明顯升高（P<0.01）；三者的錶達與腫瘤的浸潤程度有關（P<0.05），mt-P53和c-myc還與Dukes'分期和淋巴結轉移關繫密切（P<0.05）。在大腸癌中Aurora-A的暘性錶達與mt-P53、c-myc的暘性錶達呈顯著正相關（分彆為r=0.362，P<0.01；r=0.487，P<0.01），mt-P53和c-myc之間也存在顯著正相關（r=0.242，P<0.01）。結論：Aurora-A和c-myc蛋白的過錶達與P53的突變在大腸癌的髮生、侵襲和轉移中髮揮著重要作用。綜閤分析Aurora-A、mt-P53和c-myc蛋白的錶達對大腸癌的早期診斷及預後判斷具有重要價值。
목적：연구중심체상관격매Aurora-A、돌변형P53（mt-P53）화c-myc재대장암중적표체규률，탐토기상호관계급재종류발생발전중적작용。방법：응용조직심편화면역조직화학기술（SP법）검측Aurora-A、mt-P53화c-myc재130례대장암、암방조직화정상대장조직중적표체，병결합림상병이학삼수진행종합분석。결과：Aurora-A재정상대장조직、암방조직화대장암중적양성표체솔분별위0，35%，69%。mt-P53분별위0，20%，57%。c-myc분별위0，37%，76%。여정상대장조직화암방상비，암조직중Aurora-A、mt-P53화c-myc적표체명현승고（P<0.01）；삼자적표체여종류적침윤정도유관（P<0.05），mt-P53화c-myc환여Dukes'분기화림파결전이관계밀절（P<0.05）。재대장암중Aurora-A적양성표체여mt-P53、c-myc적양성표체정현저정상관（분별위r=0.362，P<0.01；r=0.487，P<0.01），mt-P53화c-myc지간야존재현저정상관（r=0.242，P<0.01）。결론：Aurora-A화c-myc단백적과표체여P53적돌변재대장암적발생、침습화전이중발휘착중요작용。종합분석Aurora-A、mt-P53화c-myc단백적표체대대장암적조기진단급예후판단구유중요개치。
Objective:This study aimed to investigate the expression levels of centrosome-associated kinase Aurora-A, mutant type P53 (mt-P53), and c-myc in colorectal cancer. This study was also conducted to investigate the mutual relationship and functions of these factors in tumorigenesis and tumor progression. Methods:We examined the pathological specimens obtained from colorectal cancer, pericancerous tissues, and normal colorectal tissues by tissue microarray technique and immunohistochemistry (SP method) to determine the expression levels of Aurora-A, mt-P53, and c-myc proteins. The clinicopathological parameters were then analyzed. Re-sults: The positive rates of Aurora-A expression in normal colorectal tissues, pericancerous tissues, and colorectal cancer were 0%, 35%, and 69%respectively;by comparison, the positive rates of mt-P53 were 0%, 20%, and 57%, respectively. For c-myc, the positive rates were 0, 37%, and 76%, respectively. The expression levels of Aurora-A, mt-P53, and c-myc were significantly higher in tumor tis-sues than in normal colorectal tissues and pericancerous tissues (P<0.01). Aurora-A overexpression was related to the depth of invasion (P<0.05). Mt-P53 and c-myc overexpression was related to the depth of invasion, lymph node metastasis, and Dukes' classification (P<0.05). A strong positive correlation was observed between the expressions of Aurora-A, mt-P53, and c-myc in colorectal cancer (r=0.362, P<0.01; r=0.487, P<0.01). A strong positive correlation was also observed between the expressions of mt-P53 and c-myc in colorectal cancer (r=0.242, P<0.01). Conclusion:The overexpression of Aurora-A and c-myc and the mutation of P53 were important in tumorigenesis, tumor invasion, and metastasis of colorectal cancer. Thus, the co-detection of Aurora-A, mt-P53, and c-myc may be useful for the early diagnosis and prognosis of colorectal cancer.
