CAJ | 학술논문

目的：探讨塑胶包埋骨髓活检切片在骨髓增生异常综合征（MDS）诊断中的价值。方法使用乙二醇甲基丙烯酸酯（GMA）包埋骨髓活检标本，在HGF和Gomori染色下观察125例MDS骨髓切片中的病态造血和间质细胞异常反应。并以25例健康人作对照。结果活检切片可正确判定骨髓增生程度，19例（15．2％）显示幼红细胞簇异位。103例低危组病例中的64例（62％）显示幼稚前体细胞（原始和早幼粒细胞）异常定位[ALIP（＋）]。单位面积内的巨核细胞和肥大细胞计数均高于对照组。Gomori染色65例示中度增加，其中10例（8．0％）骨纤程度达＋＋＋，属纤维增生型MDS。125例MDS中33例（26．4％）的骨髓活检切片内，可见红系和粒系幼稚细胞的骨髓静脉窦浸润现象。结论该研究证明，骨髓活检GM A包埋切片有助于MDS的诊断，且具有重要的临床应用价值。
목적：탐토소효포매골수활검절편재골수증생이상종합정（MDS）진단중적개치。방법사용을이순갑기병희산지（GMA）포매골수활검표본，재HGF화Gomori염색하관찰125례MDS골수절편중적병태조혈화간질세포이상반응。병이25례건강인작대조。결과활검절편가정학판정골수증생정도，19례（15．2％）현시유홍세포족이위。103례저위조병례중적64례（62％）현시유치전체세포（원시화조유립세포）이상정위[ALIP（＋）]。단위면적내적거핵세포화비대세포계수균고우대조조。Gomori염색65례시중도증가，기중10례（8．0％）골섬정도체＋＋＋，속섬유증생형MDS。125례MDS중33례（26．4％）적골수활검절편내，가견홍계화립계유치세포적골수정맥두침윤현상。결론해연구증명，골수활검GM A포매절편유조우MDS적진단，차구유중요적림상응용개치。
Objective To evaluate the value of plastic-embedded bone marrow (BM ) biopsy sections in diagnosing myelodysplas-tic syndrome(MDS) .Methods HGF and Gomori stained .Glycol methacrylate(GMA) embedded BM biopsy specimens were detec-ted from 125 MDS patients with 25 cases in good health as control .Dyshematopoiesis and abnormal stroma cell responses were se-lected as main observed projects .Results Bone marrow biopsy sections gave an exact assessment of cellularity ,19 cases (15 .2% ) showed a decrease of cellularity as hypocellular MDS .66 cases(52 .8% ) of these cases did show abnormal localization of erythro-blastic clusters ,biopsy of 64(62% ) of low risk group 103 cases did show abnormal localization of immature precursors such as blasts and promyelocytes(ALIP positive) .Numeration of megakaryocytes and mast cells within 1mm2 acre of BM tissue were all much in MDS group than in control group .Gomori′s stain revealed moderate increase in reticulin fibres in 65 cases ,10 cases(8 .0% ) showed degree of myelofibrosis were + + + as hyperfibrotic MDS .Among 125 cases ,33 cases(26 .4% ) of bone marrow biopsies showed different types of erythrocytic and granulocytic immature cells with unusual intrasinusoidal bone marrow infiltration .Con-clusion It is clear that GMA embedded BM biopsy section is helpful in making diagnosis of MDS ,and has important clinical appli-cation value .