CAJ | 학술논문

以烟草青枯病原菌[茄科雷尔氏菌(Ralstonia solanacearum)]为筛选指示菌,采用平板共培养初筛和发酵上清滤液复筛的方法,筛选到烟草青枯病拮抗菌株XLA03,其抑菌圈直径为13.74 mm；经16S rDNA鉴定,菌株XLA03为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。由单因素试验和正交试验优化得到了菌株 XLA03生物量较高的摇瓶发酵培养基配方为玉米淀粉15.0 g∕L、豆粕50.0 g∕L、K2HPO4?3H2O 1.0 g∕L、MgSO4?7H2O 0.75 g∕L、MnSO4?H2O 0.010 g∕L；培养条件为起始pH 7.0,接种量1%,装液量20 mL∕250 mL。
이연초청고병원균[가과뢰이씨균(Ralstonia solanacearum)]위사선지시균,채용평판공배양초사화발효상청려액복사적방법,사선도연초청고병길항균주XLA03,기억균권직경위13.74 mm；경16S rDNA감정,균주XLA03위해정분아포간균(Bacillus amyloliquefaciens)。유단인소시험화정교시험우화득도료균주 XLA03생물량교고적요병발효배양기배방위옥미정분15.0 g∕L、두박50.0 g∕L、K2HPO4?3H2O 1.0 g∕L、MgSO4?7H2O 0.75 g∕L、MnSO4?H2O 0.010 g∕L；배양조건위기시pH 7.0,접충량1%,장액량20 mL∕250 mL。
One bacterial strain was screened for its antagonistic activity against Ralstonia solanacearum based on the size of the bacteriostatic circle in vitro by the dual culture and culture filtrate tests,and named as XLA03. Its bacteriostatic circle could reach 13 mm. According to determination and analysis of 16S rDNA, the strain XLA03 was identified as Bacillus amyloliquefaciens. Based on the single factor experiments and orthogonal experiments, an optimal fermentation medium for its biomass-production in flasks was composed of maize starch 15.0 g∕L, soybean meal 50.0 g∕L,K2HPO4?3H2O 1.0 g∕L,MgSO4?7H2O 0.75 g∕L,MnSO4?H2O 0.01 g∕L. The optimal fermentation condition was initial pH of 7.0, inoculum size of 1% loaded liquid of 20 mL∕250 mL.