CAJ | 학술논문

为了研究活化卵白蛋白对断奶仔猪结肠内短链脂肪酸（short chain fatty acid，SCFA）的影响，选取54头20日龄健康仔猪，随机分为3个处理组。23日龄开始，Ⅰ组饲喂基础日粮＋活化卵白蛋白10 mL?d-1?头-1，Ⅱ组饲喂基础日粮＋啤酒酵母10 mL?d-1?头-1，Ⅲ组连续饲喂基础日粮10 d，28日龄断奶。仔猪在23，28，35，42日龄时，从每个重复中随机选1头屠宰，迅速结扎结肠两端，无菌收集结肠食糜，利用气相色谱法测定结肠内SCFA含量。试验结果表明：（1）仔猪28日龄时，与Ⅲ组相比，Ⅰ组仔猪结肠内乙酸（P＜0.01）、丙酸（P＜0.05）、丁酸（P＜0.05）和总SCFA（P＜0.01）含量升高，而异丁酸（P＜0.01）含量降低；与Ⅱ组相比，Ⅰ组仔猪结肠内乙酸（P＜0.01）、异丁酸（P＜0.01）和总SCFA（P＜0.01）含量升高；（2）仔猪42日龄时，与Ⅱ组、Ⅲ组相比，Ⅰ组仔猪结肠内乙酸、丙酸、丁酸、异戊酸和总SCFA含量显著升高（P＜0.05）；（3）随着仔猪日龄的增加，各组仔猪结肠内总SCFA含量均增加，但Ⅰ组增加最明显。由此看来，活化卵白蛋白可以提高断奶仔猪结肠内SCFA含量。
위료연구활화란백단백대단내자저결장내단련지방산（short chain fatty acid，SCFA）적영향，선취54두20일령건강자저，수궤분위3개처리조。23일령개시，Ⅰ조사위기출일량＋활화란백단백10 mL?d-1?두-1，Ⅱ조사위기출일량＋비주효모10 mL?d-1?두-1，Ⅲ조련속사위기출일량10 d，28일령단내。자저재23，28，35，42일령시，종매개중복중수궤선1두도재，신속결찰결장량단，무균수집결장식미，이용기상색보법측정결장내SCFA함량。시험결과표명：（1）자저28일령시，여Ⅲ조상비，Ⅰ조자저결장내을산（P＜0.01）、병산（P＜0.05）、정산（P＜0.05）화총SCFA（P＜0.01）함량승고，이이정산（P＜0.01）함량강저；여Ⅱ조상비，Ⅰ조자저결장내을산（P＜0.01）、이정산（P＜0.01）화총SCFA（P＜0.01）함량승고；（2）자저42일령시，여Ⅱ조、Ⅲ조상비，Ⅰ조자저결장내을산、병산、정산、이무산화총SCFA함량현저승고（P＜0.05）；（3）수착자저일령적증가，각조자저결장내총SCFA함량균증가，단Ⅰ조증가최명현。유차간래，활화란백단백가이제고단내자저결장내SCFA함량。
To investigate the effects of active egg white protein on colonic short chain fatty acid (SCFA) of weaned piglets, fifty four 20-day-old piglets were randomly divided into three groups , with three replications per group and six piglets each replication.Piglets in groupⅢwere fed basal diet, the group Ⅰand group Ⅱrespectively were fed basal diet supplemented with active egg white protein 10 mL?d-1 each piglet and Saccharomyces cerevisiae 10 mL?-1 each piglet.From the beginning of 23-day-old, the piglets in group Ⅰ and group Ⅱ were added to feed the active egg white protein and Saccharomyces cerevisiae for 10 days, and 28-day-old piglets were weaned.On 23, 28, d 35 and 42-day-old, a piglet randomly selected from each replication was slaughtered , both ends of colon were ligated quickly and the colonic digesta were collected aseptically to determine colonic SCFA by gas chromatography .The results showed that: (1) On 28-day-old, compared with those in group Ⅲ piglets, the colonic acetic acid ( P<0.01), propionic acid(P<0.05), butyric acid(P<0.05) and total SCFA(P<0.01) concentrations in group Ⅰpiglets increased, isobutyric acid concentration in group Ⅰ piglets decreased(P<0.01); compared with those in groupⅡpiglets, the colonic acetic acid (P <0.01), isobutyric acid ( P <0.01 ) and total SCFA ( P <0.01 ) concentrations in group Ⅰ piglets increased; (2) On 42-day-old, compared with those in group Ⅱ and group Ⅲpiglets, the colonic acetic acid(P<0.05), propionic acid(P<0.05), butyric acid(P<0.05), isovaleric acid(P<0.05) and total SCFA(P<0.05) concentrations in group Ⅰ piglets increased; (3) With the increase of piglet age, total SCFA showed a increasing tendency in every group , but groupⅠwas most obvious.It followed that active egg white protein could improve colonic SCFA concentration of weaned piglets .