CAJ | 학술논문

以克新8号为试验材料，通过优化超低温保存马铃薯茎尖中影响成活及再生的几个关键因素，成功建立了基于玻璃化法的马铃薯茎尖超低温保存体系。茎尖在MS+0.3M蔗糖的培养基上预培养1天后，经2M甘油+0.4M蔗糖的加载液室温加载30 min，浸入到PVS2溶液中（00C）处理60 min，将处理后的茎尖转至含有0.05 mg/L GA3的MS后培养基上培养，成活率和再生率达到94.8%和78.1%。并将建立的玻璃化法应用到其它4个品种，平均再生率为65.5%，研究结果可为中国马铃薯种质资源的超低温保存提供技术支持。
이극신8호위시험재료，통과우화초저온보존마령서경첨중영향성활급재생적궤개관건인소，성공건립료기우파리화법적마령서경첨초저온보존체계。경첨재MS+0.3M자당적배양기상예배양1천후，경2M감유+0.4M자당적가재액실온가재30 min，침입도PVS2용액중（00C）처리60 min，장처리후적경첨전지함유0.05 mg/L GA3적MS후배양기상배양，성활솔화재생솔체도94.8%화78.1%。병장건립적파리화법응용도기타4개품충，평균재생솔위65.5%，연구결과가위중국마령서충질자원적초저온보존제공기술지지。
in order to Kexin 8 as experimental material, through the optimization of cryopreservation of several key factors affecting potato shoot tip survival and regeneration, successfully established the potato meristem cryopreservation by vitrification based system. Cul-ture of shoot tip in MS+0.3M sucrose medium pre cultured for 1 days, the loading solution at room temperature loading 2M glycerol+0.4M sucrose 30 min, immersed in PVS2 solution (00C) treatment for 60 min, the stem tip to treated with 0.05 mg/L GA3 after MS culture medi-um, the survival rate and regeneration rate 94.8%and 78.1%. Application for vitrification method was set up to 4 species of other, average regeneration rate was 65.5%, the research results can be used for cryopreservation of potato germplasm resources China provide technical support.