CAJ | 학술논문

目的：观察阿托伐他汀对ox-LDL诱导U937细胞形成泡沫细胞过程中Lkn-1表达的影响，探讨阿托伐他汀抗AS的作用机制。方法在由0．1μmol/L佛波脂（ PMA）诱导分化人U937巨噬细胞中加入100 mg/Lox-LDL及不同浓度（0．1、1、10μmol/L）的阿托伐他汀共同孵育24 h，分别用酶联免疫吸附试验（ELISA）和RT-PCR方法检测培养细胞上清中Lkn-1的表达变化。结果 ox-LDL组较正常对照组Lkn-1的表达明显增加（ P ＜0．05）。给药各组较ox-LDL组Lkn-1明显减少（ P ＜0．05）。且随阿托伐他汀浓度的增加Lkn-1表达呈逐渐减少的趋势（ P ＜0．05）。结论阿托伐他汀能抑制ox-LDL诱导U937细胞系形成泡沫细胞过程炎症因子Lkn-1的表达和分泌，可能为其抗动脉粥样硬化的重要机制之一。
목적：관찰아탁벌타정대ox-LDL유도U937세포형성포말세포과정중Lkn-1표체적영향，탐토아탁벌타정항AS적작용궤제。방법재유0．1μmol/L불파지（ PMA）유도분화인U937거서세포중가입100 mg/Lox-LDL급불동농도（0．1、1、10μmol/L）적아탁벌타정공동부육24 h，분별용매련면역흡부시험（ELISA）화RT-PCR방법검측배양세포상청중Lkn-1적표체변화。결과 ox-LDL조교정상대조조Lkn-1적표체명현증가（ P ＜0．05）。급약각조교ox-LDL조Lkn-1명현감소（ P ＜0．05）。차수아탁벌타정농도적증가Lkn-1표체정축점감소적추세（ P ＜0．05）。결론아탁벌타정능억제ox-LDL유도U937세포계형성포말세포과정염증인자Lkn-1적표체화분비，가능위기항동맥죽양경화적중요궤제지일。
Objetc ive To observe the effect of atorvastatin ( AT) on the expression of Lkn-1 during U937 monocyte differentiating into foam cells induced by oxidized LDL ( ox-LDL) ,and to explore the antiatherosclerotic action mechanism of AT .Method s The macrophages derived from U 937 monocytes were induced by PMA (0.1μmol/L),which were treated with 100mg/L ox-LDL plus different concentrations of AT (0.1,1,10μmol/L) for 24 hours.Then the expression levels of Lkn-1 in supernatant of cultured cell were detected by ELISA and RT-PCR.Results As compared with those in control group ,the expression levels of Lkn-1 of in ox-LDL group were significantly increased ( P <0.05).However the expression levels of Lkn-1 in different concentrations of AT groups were significantly decreased ,as compared with those in ox-LDL group ( P <0.05).Moreover with the increase of AT concentration, the the expression levels of Lkn-1 were gradually decreased ( P <0.05 ). Conclusion Atorvastatin can inhibit the expression and secretion of Lkn-1 in the course of U937 monocyte differentiating into foam cells induced by ox-LDL,which may be the action mechanism of AT in anti-atherosclerosis and in inhibiting inflammatory factor .