CAJ | 학술논문

目的：通过观察雌激素对去势雌鼠血清及膀胱e-NOS、AQP1含量的影响，进一步探讨雌激素替代治疗对绝经后尿道综合征的机制。方法成年健康SD雌性大鼠24只，随机分为空白组、对照组、尼尔雌醇组。摘除卵巢建立大鼠雌激素缺乏模型，灌胃给药4周后，用ELISA法测定血清及膀胱组织中e-NOS、AQP1的含量。结果尼尔雌醇组与对照组比较，血清e-NOS、AQP1含量显著增高（P<0.05）；与空白组比较，差异无统计学意义（P＞0.05）。去势后膀胱e-NOS明显下降，补充雌激素后恢复正常水平，而AQP1去势前、后无显著改变，补充雌激素后亦无明显改变趋势。结论卵巢切除导致大鼠膀胱e-NOS发生下降，补充雌激素后恢复，而AQP1无差异。e-NOS在绝经后尿道综合征中起到直接作用。
목적：통과관찰자격소대거세자서혈청급방광e-NOS、AQP1함량적영향，진일보탐토자격소체대치료대절경후뇨도종합정적궤제。방법성년건강SD자성대서24지，수궤분위공백조、대조조、니이자순조。적제란소건립대서자격소결핍모형，관위급약4주후，용ELISA법측정혈청급방광조직중e-NOS、AQP1적함량。결과니이자순조여대조조비교，혈청e-NOS、AQP1함량현저증고（P<0.05）；여공백조비교，차이무통계학의의（P＞0.05）。거세후방광e-NOS명현하강，보충자격소후회복정상수평，이AQP1거세전、후무현저개변，보충자격소후역무명현개변추세。결론란소절제도치대서방광e-NOS발생하강，보충자격소후회복，이AQP1무차이。e-NOS재절경후뇨도종합정중기도직접작용。
Objective To investigate the effect of hormonal alteration on the expression of e-NOS and AQP1 in menopausal rat urinary bladder and serum, and illustrate the mechanism of estrogen replacement for post-menopause urethral syndrome. Methods Female rats(N=24) were divided into three groups:control, bilateral ovariectomy(Ovx), and bilateral ovariectomy followed by feeding 17β-estradiol(Ovx+Est). After 4 weeks, the expressions of e-NOS and AQP1 in the bladder and in the serum were determined by Enzyme Linked Immunosorbent Assay. Results The expressions of AQP1 in bladder of three groups have no significant difference in bladder, but AQP1 in serum of Ovx+Est group was higher than control and Ovx group. The e-NOS expressions ware significantly decreased in the Ovx group and restored to the control level in the Ovx+Est group in bladder and also in serum. Conclusions This research suggested that ovariectomy cause a significant change in eNOS expression but not in the AQP1 expression in menopausal rat urinary bladder. These imply that e-NOS but not AQP1have a direct function in the urethral syndrome after menopause.