CAJ | 학술논문

目的：观察过表达UCA1基因、UCA1a(CUDR)基因对人膀胱癌UM-UC-2细胞周期的影响。方法以前期实验中构建好的3组细胞[转染pcDNA-UCA1重组表达载体的UM-UC-2细胞、转染pcDNA-UCA1a(CUDR)重组表达载体的UM-UC-2细胞和转染空质粒pcDNA3.1(＋)的UM-UC-2细胞（对照组）]作为材料，采用流式细胞术检测UCA1基因、UCA1a(CUDR)基因对UM-UC-2细胞周期的影响。结果与对照组相比，转染pcDNA-UCA1重组表达载体的UM-UC-2细胞、转染pcDNA-UCA1a(CUDR)重组表达载体的UM-UC-2细胞S期的细胞百分比均明显增加，G0/G1期的细胞百分比均明显减少。结论过表达UCA1基因、UCA1a(CUDR)基因均能够促进人膀胱癌UM-UC-2细胞的DNA合成，使UM-UC-2细胞增殖能力增强。
목적：관찰과표체UCA1기인、UCA1a(CUDR)기인대인방광암UM-UC-2세포주기적영향。방법이전기실험중구건호적3조세포[전염pcDNA-UCA1중조표체재체적UM-UC-2세포、전염pcDNA-UCA1a(CUDR)중조표체재체적UM-UC-2세포화전염공질립pcDNA3.1(＋)적UM-UC-2세포（대조조）]작위재료，채용류식세포술검측UCA1기인、UCA1a(CUDR)기인대UM-UC-2세포주기적영향。결과여대조조상비，전염pcDNA-UCA1중조표체재체적UM-UC-2세포、전염pcDNA-UCA1a(CUDR)중조표체재체적UM-UC-2세포S기적세포백분비균명현증가，G0/G1기적세포백분비균명현감소。결론과표체UCA1기인、UCA1a(CUDR)기인균능구촉진인방광암UM-UC-2세포적DNA합성，사UM-UC-2세포증식능력증강。