中国执业药师
中國執業藥師
중국집업약사
CHINA LICENSED PHARMACIST
2014年
1期
16-19
,共4页
灵芝孢子粉%多糖%制备工艺
靈芝孢子粉%多糖%製備工藝
령지포자분%다당%제비공예
Ganoderma Lucidum Spore%Polysaccharide%Preparation Technology
目的：针对灵芝孢子粉多糖的制备工艺进行研究。方法：以多糖转移率为指标，比较布袋包煎和搅拌提取两种工艺；采用单因素法，考察搅拌提取中料液比和提取次数对多糖提取的影响；根据滤液的性状，比较静置取上清液、离心、布袋滤过和板框滤过等多种滤过方法，优化滤过工艺。结果：灵芝孢子粉多糖的最佳制备工艺条件为：按料液比1∶30加水至提取罐中，煮至100℃，投料，保温搅拌提取2 min ，静置24 min ，取上清液，离心，滤液先通过（5μm 滤袋），再板框滤过，滤液浓缩至相对密度为1.10（60℃），醇沉，取沉淀减压干燥，干膏得率为3.26%，多糖含量为25.1%，多糖转移率97%。结论：该工艺可行有效，具有实际生产意义。
目的：針對靈芝孢子粉多糖的製備工藝進行研究。方法：以多糖轉移率為指標，比較佈袋包煎和攪拌提取兩種工藝；採用單因素法，攷察攪拌提取中料液比和提取次數對多糖提取的影響；根據濾液的性狀，比較靜置取上清液、離心、佈袋濾過和闆框濾過等多種濾過方法，優化濾過工藝。結果：靈芝孢子粉多糖的最佳製備工藝條件為：按料液比1∶30加水至提取罐中，煮至100℃，投料，保溫攪拌提取2 min ，靜置24 min ，取上清液，離心，濾液先通過（5μm 濾袋），再闆框濾過，濾液濃縮至相對密度為1.10（60℃），醇沉，取沉澱減壓榦燥，榦膏得率為3.26%，多糖含量為25.1%，多糖轉移率97%。結論：該工藝可行有效，具有實際生產意義。
목적：침대령지포자분다당적제비공예진행연구。방법：이다당전이솔위지표，비교포대포전화교반제취량충공예；채용단인소법，고찰교반제취중료액비화제취차수대다당제취적영향；근거려액적성상，비교정치취상청액、리심、포대려과화판광려과등다충려과방법，우화려과공예。결과：령지포자분다당적최가제비공예조건위：안료액비1∶30가수지제취관중，자지100℃，투료，보온교반제취2 min ，정치24 min ，취상청액，리심，려액선통과（5μm 려대），재판광려과，려액농축지상대밀도위1.10（60℃），순침，취침정감압간조，간고득솔위3.26%，다당함량위25.1%，다당전이솔97%。결론：해공예가행유효，구유실제생산의의。
Objective: To study the preparation technology of polysaccharide extracted from ganoderma lucidum spore . Methods: The transfer rate of polysaccharide was used as an index to compare the processes of cloth bag decoction and stirring extractions . By the single factor method , the effect of extraction times and the ratio of water to material on the yield of polysaccharide extracted from spores of granoderma atrum were investigated . According to properties of filtrate the preparation methods were studied including static supernatant extraction , centrifugation , bag filtration and plate and frame filter so as to optimize the filtration technology . Results: The optimized preparation conditions for polysaccharide of ganoderma lucidum spore were as follows:add water to the extraction pot according to the material liquid ratio 1∶30 , heating to 100 ℃, then adding the material , stirring with heat preservation and extraction for 2 min , standing for 24 min , collect the the supernatant , centrifuge , filtrate through ( 5 μm bag ) and plate and frame filter , concentrate the filtrate to a relative density of 1 . 10 ( 60 ℃) , alcohol precipitation , collect the precipitate and vacuum drying , the dry extract rate was 3 . 26%, polysaccharide content was 25 . 1%, and transfer rate of polysaccharide was 97%. Conclusion: The process is feasible and effective and has practical significance to production .
