浙江中西医结合杂志
浙江中西醫結閤雜誌
절강중서의결합잡지
ZHEJIANG JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2014年
1期
16-17
,共2页
斯伟宏%卢根林%黄惊鸿%吴爱兵
斯偉宏%盧根林%黃驚鴻%吳愛兵
사위굉%로근림%황량홍%오애병
大鼠%缺血-再灌注损伤%硫化氢%凝血功能
大鼠%缺血-再灌註損傷%硫化氫%凝血功能
대서%결혈-재관주손상%류화경%응혈공능
rats%ischemia-reperfusion injury%hydrogen sulfide%coagulation function
目的：研究血硫化氢（H2S）浓度与肠缺血-再灌注损伤大鼠凝血功能的相关性。方法雄性Wistar大鼠24只，分为S（假手术）组、I（缺血-再灌注）组，N（缺血-再灌注+硫氢化钠（NaHS））组，每组8只。S组仅行剖腹及游离肠系膜上动脉（SMA）术，I和N组剖腹、游离及钳夹肠SMA 60min和再灌注120min，建立大鼠肠缺血-再灌注损伤模型。N组于再灌注前10min静脉注射NaHS 100μmol/kg后，按1mg/（kg·h)持续静脉注射直到再灌注2h，S和I组静脉注射相同体积的生理盐水，作用时间和持续时间均同N组。取血测定H2S和凝血功能指标。结果 N组PT、APTT、TT、D-二聚体均低于I组、高于S组（P约0.01），N组H2S、FIB低于S组、高于I组（P约0.01）。H2S与FIB呈正相关（r=0.758，P约0.01），与PT、APTT、TT、D-二聚体呈负相关（r=-0.715、-0.721、-0.689、-0.748，P约0.01）。结论血H2S与肠缺血-再灌注损伤大鼠凝血功能改善相关。
目的：研究血硫化氫（H2S）濃度與腸缺血-再灌註損傷大鼠凝血功能的相關性。方法雄性Wistar大鼠24隻，分為S（假手術）組、I（缺血-再灌註）組，N（缺血-再灌註+硫氫化鈉（NaHS））組，每組8隻。S組僅行剖腹及遊離腸繫膜上動脈（SMA）術，I和N組剖腹、遊離及鉗夾腸SMA 60min和再灌註120min，建立大鼠腸缺血-再灌註損傷模型。N組于再灌註前10min靜脈註射NaHS 100μmol/kg後，按1mg/（kg·h)持續靜脈註射直到再灌註2h，S和I組靜脈註射相同體積的生理鹽水，作用時間和持續時間均同N組。取血測定H2S和凝血功能指標。結果 N組PT、APTT、TT、D-二聚體均低于I組、高于S組（P約0.01），N組H2S、FIB低于S組、高于I組（P約0.01）。H2S與FIB呈正相關（r=0.758，P約0.01），與PT、APTT、TT、D-二聚體呈負相關（r=-0.715、-0.721、-0.689、-0.748，P約0.01）。結論血H2S與腸缺血-再灌註損傷大鼠凝血功能改善相關。
목적：연구혈류화경（H2S）농도여장결혈-재관주손상대서응혈공능적상관성。방법웅성Wistar대서24지，분위S（가수술）조、I（결혈-재관주）조，N（결혈-재관주+류경화납（NaHS））조，매조8지。S조부행부복급유리장계막상동맥（SMA）술，I화N조부복、유리급겸협장SMA 60min화재관주120min，건립대서장결혈-재관주손상모형。N조우재관주전10min정맥주사NaHS 100μmol/kg후，안1mg/（kg·h)지속정맥주사직도재관주2h，S화I조정맥주사상동체적적생리염수，작용시간화지속시간균동N조。취혈측정H2S화응혈공능지표。결과 N조PT、APTT、TT、D-이취체균저우I조、고우S조（P약0.01），N조H2S、FIB저우S조、고우I조（P약0.01）。H2S여FIB정정상관（r=0.758，P약0.01），여PT、APTT、TT、D-이취체정부상관（r=-0.715、-0.721、-0.689、-0.748，P약0.01）。결론혈H2S여장결혈-재관주손상대서응혈공능개선상관。
Objective To investigate the effect of hydrogen sulfide on coagulation function during rodent gut is-chemia-reperfusion injury. Methods Twenty-four Wistar rats were randomly divided into sham, ischemia-reperfusion (IR), ischemia-reperfusion+sodium hydrosulfide(N) groups(n=8). Rats in Group N received sodium hydrosulfide(100μmol/kg bolus+1 mg.kg-1·h-1 infusion) 10 min prior to the onset of reperfusion (cut off blood supply to superior mesenteric artery for 60 min and then recover blood supply for 120 min), whereas rats in Group S and IR received normal sodium of equal volume. Group S only received abdominal incision and dissociation of superior mesenteric artery. Serum hydrogen sulfide and haemorheological parameter were determined. Results PT, APTT, TT, and D-dimer of group N were predominantly higher than those in Group S, but strikingly lower than those in Group I (P<0.01). Hydrogen sulfide and FIB in Group N were significantly higher than those in Group I, but lower than those in Group S(P<0.01). Hydrogen sulfide was positively correlated with FIB(r=0.758, P<0.01), but negatively correlated with PT, APTT, TT, and D-dimer (r=-0.715, -0.721, -0.689, -0.748, P<0.01). Conclusion Treatment with Hydro-gen sulfide can have a protective effect on coagulation function during gut ischemia-reperfusion injury in rats.
