CAJ | 학술논문

目的：研究七叶皂苷钠对人急性单核白血病细胞株SHI-1细胞的抑制增殖及诱导凋亡的作用。方法 SHI-1细胞经不同浓度的七叶皂苷钠处理后，MTT法分析七叶皂苷钠对SHI-1细胞增殖的抑制作用，Annexin吁/PI染色流式细胞术（FCM）检测细胞凋亡率，DNA凝胶电泳观察凋亡特异性DNA降解梯形条带，半定量PCR检测凋亡特异性酶Caspase-3 mRNA表达水平。结果MTT法显示七叶皂苷钠能显著抑制SHI-1细胞的增殖，呈时间和剂量依赖性（P<0.05）。经不同浓度的七叶皂苷钠处理24h，Annexin吁/PI染色显示Annexin吁阳性的凋亡细胞率明显上升，DNA琼脂糖凝胶电泳分析出现凋亡特异性的DNA降解梯形条带，半定量PCR显示Caspase-3 mRNA表达水平随药物浓度增加而升高（P<0.05）。结论七叶皂苷钠对SHI-1细胞具有抑制增殖和诱导凋亡的作用，可能是其抗白血病作用途径之一。
목적：연구칠협조감납대인급성단핵백혈병세포주SHI-1세포적억제증식급유도조망적작용。방법 SHI-1세포경불동농도적칠협조감납처리후，MTT법분석칠협조감납대SHI-1세포증식적억제작용，Annexin우/PI염색류식세포술（FCM）검측세포조망솔，DNA응효전영관찰조망특이성DNA강해제형조대，반정량PCR검측조망특이성매Caspase-3 mRNA표체수평。결과MTT법현시칠협조감납능현저억제SHI-1세포적증식，정시간화제량의뢰성（P<0.05）。경불동농도적칠협조감납처리24h，Annexin우/PI염색현시Annexin우양성적조망세포솔명현상승，DNA경지당응효전영분석출현조망특이성적DNA강해제형조대，반정량PCR현시Caspase-3 mRNA표체수평수약물농도증가이승고（P<0.05）。결론칠협조감납대SHI-1세포구유억제증식화유도조망적작용，가능시기항백혈병작용도경지일。
Objective To investigate the effects of sodium aescinate on inhibiting proliferation and inducing apoptosis of human acute monocytic leukemic SHI-1 cells. Methods Proliferation of sodium aescinate treated SHI-1 cells was detected by MTT method. The apoptosis rates of SHI-1 cells were analyzed by flow cytometry af-ter annexin V/PI staining. The gel electrophoresis of DNA ladder was used to analyze the bands of DNA degrada-tion, and the expression level of caspase-3 mRNA was tested by reverse transcription-polymerase chain reaction (RT-PCR). Results MTT assay showed that the proliferation of SHI-1 cells was effectively inhibited by sodium aescinate in a time-and-does dependent manner(P<0.05). The Annexinⅴ positive rate of SHI-1 cells after treated with sodium aescinate was significantly increased by FCM analysis(P<0.05). The apoptosis typical DNA fragments of SHI-1 cells treated with sodium aescinate was presented on the gel electrophoresis. The expression level of cas-pase-3 mRNA was up-regulated with increasing concentration of sodium aescinate by RT-PCR detection(P<0.05). Con-clusion Treatment with sodium aescinate can have inhibitory effect on proliferation and induce apoptosis of SHI-1 leukemia cells. Our results provides an experimental evidence for the activity of sodium aescinate on anti-leukemia.