检验医学
檢驗醫學
검험의학
LABORATORY MEDICINE
2014年
5期
553-555
,共3页
田鼠巴贝斯虫%疟原虫%感染%诊断
田鼠巴貝斯蟲%瘧原蟲%感染%診斷
전서파패사충%학원충%감염%진단
Babesia%Plasmodium%Infection%Diagnosis
目的：通过回顾1例人感染巴贝斯虫的实验室资料，以积累经验，提高诊断水平。方法研究红细胞（RBC）、血小板（PLT）直方图，白细胞（WBC）VCS散点图；观察外周血、骨髓涂片中虫体的形态；从患者外周血中提取的DNA核酸用疟原虫、巴贝斯虫种、巴贝斯虫属特异性引物进行聚合酶链反应（PCR）。结果 RBC直方图波峰降低，宽度增加，PLT直方图无异常，WBC VCS散点图非WBC区有颗粒团；外周血、骨髓涂片见大量拟似恶性疟原虫虫体；PCR产物测序结果经BLAST比对，结果显示与巴贝斯虫和田鼠巴贝斯虫18s核糖体DNA序列分别有96％和99％的同源性。结论感染的虫体为田鼠巴贝斯虫。
目的：通過迴顧1例人感染巴貝斯蟲的實驗室資料，以積纍經驗，提高診斷水平。方法研究紅細胞（RBC）、血小闆（PLT）直方圖，白細胞（WBC）VCS散點圖；觀察外週血、骨髓塗片中蟲體的形態；從患者外週血中提取的DNA覈痠用瘧原蟲、巴貝斯蟲種、巴貝斯蟲屬特異性引物進行聚閤酶鏈反應（PCR）。結果 RBC直方圖波峰降低，寬度增加，PLT直方圖無異常，WBC VCS散點圖非WBC區有顆粒糰；外週血、骨髓塗片見大量擬似噁性瘧原蟲蟲體；PCR產物測序結果經BLAST比對，結果顯示與巴貝斯蟲和田鼠巴貝斯蟲18s覈糖體DNA序列分彆有96％和99％的同源性。結論感染的蟲體為田鼠巴貝斯蟲。
목적：통과회고1례인감염파패사충적실험실자료，이적루경험，제고진단수평。방법연구홍세포（RBC）、혈소판（PLT）직방도，백세포（WBC）VCS산점도；관찰외주혈、골수도편중충체적형태；종환자외주혈중제취적DNA핵산용학원충、파패사충충、파패사충속특이성인물진행취합매련반응（PCR）。결과 RBC직방도파봉강저，관도증가，PLT직방도무이상，WBC VCS산점도비WBC구유과립단；외주혈、골수도편견대량의사악성학원충충체；PCR산물측서결과경BLAST비대，결과현시여파패사충화전서파패사충18s핵당체DNA서렬분별유96％화99％적동원성。결론감염적충체위전서파패사충。
Objective To review the laboratory data of one human case infected with babesia,in order to gain experience and improve the diagnosis level.Methods The histograms of red blood cell (RBC),platelet (PLT)and white blood cell(WBC)VCS scatterplot were analyzed.The parasite morphology was observed in peripheral blood and bone marrow smears.DNA extracted from peripheral blood of the patients with plasmodium vivax,voles babesia and babesia DNA-specific primers by polymerase chain reaction (PCR).Results RBC histogram peak reduced,and its width increased.PLT histogram had no abnormality.The non-WBC area of WBC VCS scatter plot had particle group. Peripheral blood and bone marrow smears emerged a large number of quasi plasmodium falciparum parasites.The results of PCR product sequencing showed that the sequencing results had 96%and 99%homology with the 18s ribosomal DNA sequence of voles babesia and babesia.Conclusions The patient is infected with voles babesia.
