CAJ | 학술논문

目的：通过应用戊巴比妥钠对10周龄的ICR小鼠实施麻醉，探索安全有效的麻醉剂量，并改良小鼠双侧卵巢切除术的围手术期处理。方法选用 SPF级ICR品系6w龄雌性小鼠60只，随机分为正常组和肝损伤组，正常组喂养标准饮食，肝损伤组喂养胆碱蛋氨酸缺乏饮食（Methionine and choline deficient diets, MCD diets）。4w后分别给予1%戊巴比妥钠80mg/kg、10mg/kg腹腔注射，对麻醉效果及术后复苏进行测试，术中术后采取保暖、伤口涂抗生素预防感染等措施，探索小鼠双侧卵巢切术围手术期改良。结果正常组和肝损伤组，1%戊巴比妥钠80mg/kg和10mg/kg可保证麻醉安全有效；术中术后给予保温，缝合时伤口涂抗生素等手段改良，可促进复苏，大大提高存活率。结论在生理和病理状态下，戊巴比妥钠的麻醉剂量不同，尤其在肝损伤情况下，麻醉剂量应大大降低且应慎用。通过保温、预防感染等手段对卵巢切除术的围手术期处理进行改良，可加快术后苏醒，提高存活率，减少术后并发症。
목적：통과응용무파비타납대10주령적ICR소서실시마취，탐색안전유효적마취제량，병개량소서쌍측란소절제술적위수술기처리。방법선용 SPF급ICR품계6w령자성소서60지，수궤분위정상조화간손상조，정상조위양표준음식，간손상조위양담감단안산결핍음식（Methionine and choline deficient diets, MCD diets）。4w후분별급여1%무파비타납80mg/kg、10mg/kg복강주사，대마취효과급술후복소진행측시，술중술후채취보난、상구도항생소예방감염등조시，탐색소서쌍측란소절술위수술기개량。결과정상조화간손상조，1%무파비타납80mg/kg화10mg/kg가보증마취안전유효；술중술후급여보온，봉합시상구도항생소등수단개량，가촉진복소，대대제고존활솔。결론재생리화병리상태하，무파비타납적마취제량불동，우기재간손상정황하，마취제량응대대강저차응신용。통과보온、예방감염등수단대란소절제술적위수술기처리진행개량，가가쾌술후소성，제고존활솔，감소술후병발증。
Objective to explore the best anesthesia dosages of sodium pentobarbital and improvement of bilateral oophorectomy through application of l0-week-old ICR mice during preoperative period. Methods ICR strains female mice in SPF of 6 weeks-age were selected for 60, randomly divided into normal group and hepatic injury group. The normal group fed a standard diets while liver injury group fed choline methionine deficient diets (Methionine and choline deficient diets, MCD diets). After 4 weeks, the mice were injected with 1% sodium pentobarbital by intraperitoneal injection. The dose was 80mg/kg and 10mg/kg respectively. The anesthetic ef ects and postoperative recovery were observed. The improved measures of insulation and coating with antibiotics in the wound to prevent infection and other measures were taken to explore the mice improved bilateral ovaries cut surgery during perioperative period. Results 1% sodium pentobarbital with 80mg/kg and 10mg/kg could ensure a safe and ef ective anesthetic. The improved measures of warming and coating with antibiotics in the wound to prevent infection and other measures were taken during and after operation, which can promote recovery and augment survival rate. Conclusion Doses of sodium pentobarbital used were dif erent in the physiological and pathological conditions, especial y in liver injury, the doses should be greatly reduced and used modestly. The methods of insulation and prevention of infection and etc. improved the bilateral ovaries cut surgery during perioperative period, accelerating the postoperative recovery, improving the survival rate and reducing the postoperative complications.