CAJ | 학술논문

纤维素乙醇的统合生物加工过程（consolidated bioprocessing，CBP）是将（半）纤维素酶生产、纤维素水解和乙醇发酵过程组合，通过一种微生物完成的生物加工过程。 CBP有利于降低生物转化过程的成本，受到研究者的普遍关注。酿酒酵母（ Saccharomyces cerevisiae）作为传统的乙醇生产菌株，是极具潜力的CBP底盘细胞。纤维小体是某些厌氧微生物细胞表面由纤维素酶系与支架蛋白组成的大分子复合物，它能高效降解木质纤维，在酿酒酵母表面展示纤维小体已成为构建CBP细胞的研究热点。笔者综述了人造纤维小体在酿酒酵母细胞表面展示组装的研究进展，重点阐述了纤维小体各元件的设计和改造，并针对酿酒酵母分泌途径的改造，提出提高人造纤维小体分泌组装的可能性策略。
섬유소을순적통합생물가공과정（consolidated bioprocessing，CBP）시장（반）섬유소매생산、섬유소수해화을순발효과정조합，통과일충미생물완성적생물가공과정。 CBP유리우강저생물전화과정적성본，수도연구자적보편관주。양주효모（ Saccharomyces cerevisiae）작위전통적을순생산균주，시겁구잠력적CBP저반세포。섬유소체시모사염양미생물세포표면유섬유소매계여지가단백조성적대분자복합물，타능고효강해목질섬유，재양주효모표면전시섬유소체이성위구건CBP세포적연구열점。필자종술료인조섬유소체재양주효모세포표면전시조장적연구진전，중점천술료섬유소체각원건적설계화개조，병침대양주효모분비도경적개조，제출제고인조섬유소체분비조장적가능성책략。
Consolidated bioprocessing ( CBP ) of lignocellulose ethanol production can combine enzyme production,cellulose hydrolysis,and fermentation into a single process by one microorganism.CBP process has gained more attention because it can reduce the costs of biotransformation processes.As a traditional ethanol producer, Saccharomyces cerevisiae is a promising CBP candidate.Cellulosome, a multi-enzyme complex,is assembled on the cell surface of some anaerobic microorganisms by various cellulases and scaffoldins.The assembly of cellulosome on S.cerevisiae has been becoming a new hot spot in CBP organism constructing research due to its high cellulose hydrolytic activity.The progresses of the assembly of cellulosome on the surface of S.cerevisiae are introduced,the design and improvement of its components are mainly summarized,and the possible strategies are analyzed to enhace the expression and assembly through engineering secretory pathway.