CAJ | 학술논문

黄翅大白蚁（ Macrotermes barneyi）具有高效降解木质纤维素的能力，其后肠内存在着丰富的共生微生物。采用活性电泳和变形梯度凝胶电泳的方法对黄翅大白蚁后肠降解滤纸微生物群落进行分析。活性电泳实验证实了此微生物群落纤维素酶的存在（内切葡聚糖酶、β葡萄糖苷酶和木聚糖酶），变形梯度凝胶电泳实验鉴定出微生物组的群落结构，即7种细菌和3种真菌。本研究初步阐明了黄翅大白蚁后肠内与滤纸降解相关的微生物种类，为进一步了解黄翅大白蚁纤维素的降解机制以及生物质资源的高效利用提供了理论基础。
황시대백의（ Macrotermes barneyi）구유고효강해목질섬유소적능력，기후장내존재착봉부적공생미생물。채용활성전영화변형제도응효전영적방법대황시대백의후장강해려지미생물군락진행분석。활성전영실험증실료차미생물군락섬유소매적존재（내절포취당매、β포도당감매화목취당매），변형제도응효전영실험감정출미생물조적군락결구，즉7충세균화3충진균。본연구초보천명료황시대백의후장내여려지강해상관적미생물충류，위진일보료해황시대백의섬유소적강해궤제이급생물질자원적고효이용제공료이론기출。
Macrotermes barneyi hindgut harbored large quantity of microbes.The native-page method and the denatured gradient gel electrophoresis were used to analyze the filter paper-degrading microbiome of M.barneyi hindgut.EG, BG and xyl were verified by the native-page method.Microbial community structure was identified by the denatured gradient gel electrophoresis.The microbial species,including 7 bacterial and 3 fungi,associated with filter paper degradation in the hindgut of M.barneyi were illustrated.The understanding of cellulose degradation mechanisms was facilitated and some theoretical basis for efficient biomass transformation was provided.